大鼠海马过表达脑源性神经营养因子对脑卒中后抑郁行为的影响

被引：16

作者：

傅晓艳 ^{[1
]}

陈浩浩 ^{[1
]}

丁明星 ^{[1
,2
]}

张宁 ^{[1
]}

方远书 ^{[3
]}

张辉 ^{[3
]}

方马荣 ^{[2
]}

机构：

[1] 金华职业技术学院医学院解剖学教研室

[2] 浙江大学医学院解剖学教研室

[3] 金华市实验动物中心

来源：

解剖学杂志 | 2015年 / 06期

关键词：

海马; 脑源性神经营养因子; 慢病毒; 脑卒中; 抑郁;

D O I：

暂无

中图分类号：

R743.3 [急性脑血管疾病（中风）]; R749.4 [情感性精神病];

学科分类号：

摘要：

目的:本研究旨在阐明脑源性神经营养因子(BDNF)过表达对脑卒中后抑郁症(PSD)大鼠抑郁样行为的影响。方法;构建BDNF过表达慢病毒载体,先结扎阻断大脑中动脉建立大鼠局灶性脑缺血模型,再采用孤养结合慢性不可预见性温和应激(CMUS)建立PSD大鼠模型。然后海马注射BDNF过表达慢病毒(PSD+LV-BDNF组),应用Real-time PCR和免疫印迹检测海马BDNF mRNA和蛋白的表达水平。结果:BDNF过表达慢病毒注射后第28天,PSD+LV-BDNF组的BDNF mRNA、蛋白表达均多于PSD组。分别于CMUS后、手术前和注射后28 d采用糖水消耗试验和旷场试验检测大鼠抑郁样行为,PSD+LV-BDNF组的糖水消耗量及水平移动次数比PSD组均显著增加。结论:在PSD大鼠海马内BDNF、的过表达可改善对脑卒中后抑郁症的抑郁样行为,发挥神经保护作用。

引用

页码：689 / 693

页数：5

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