白细胞介素6与血小板活化因子对中性粒细胞凋亡的抑制作用附视频

被引：5

作者：

管增伟

李勇

王盛兰

杨建如

马仕良

袁兰

机构：

[1] 北京医科大学医药卫生分析中心!北京

[2] 北京医科大学中国妇婴保健中心

[3] 北京医科大学病理学系

来源：

卫生研究 | 2000年 / 03期

关键词：

白细胞介素6; 血小板活化因子; 拮抗剂; 中性粒细胞; 损伤; 炎症;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

100104 ;

摘要：

应用血浆 Percoll梯度分离技术分离健康供血者的中性粒细胞 (PMN)并以 2× 10 7PMN/ml培养2 4h,实验分白细胞介素 6 (IL- 6 )、血小板活化因子 (PAF)单独处理组。 PAF受体拮抗剂 BN5 2 0 2 1预处理后再以 PAF、IL - 6分别处理组。吖啶橙和溴乙啶染色 ,荧光显微镜下观察细胞形态并记数进行细胞凋亡的定性定量分析。结果表明 ,IL- 6和 PAF均有抑制 PMN凋亡的作用。PAF受体拮抗剂 BN5 2 0 2 1能消除 IL- 6和PAF抑制 PMN凋亡的作用。提示 IL - 6抑制 PMN凋亡的机制可能是通过 PAF而实现的。从而从一个侧面说明了 IL- 6和 PAF介导的损伤后炎症反应及继发性器官功能衰竭可能与抑制中性粒细胞凋亡加剧炎症反应有关。

引用

页码：131 / 133

页数：3

共 12 条

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