蒙古黄芪的组织培养及辐射诱变研究(英文)

[Objective] The research aimed to study the tissue culture technology and callus induction by radiation mutation of A.membranaceus Bge.[Method] With the different parts of Astragalus membranaceus Bge.var.mongholicus(Bge.) Hsiao aseptic seedling as explants(leaves,cotyledons,hypocotyls) induced callus,and cotyledon and hypocotyls taken by the method of radiation mutation were studied.[Result]The results showed that the three explants had relatively high callus induced rate in the medium which respectively made up of MS +6-BA 2.0 mg/L + NAA 2.0 mg/L,LS +6-BA 2.0 mg/L +NAA 0.1 mg/L,MS + 6-BA 2.0 mg/L + NAA 2.0 mg/L;the optimum mutation time of hypocotyls and cotyledons was 15 minutes;the growth of the callus induced from hypocotyls would be better as the mutation time increased,but when it reached a certain time the growth would be weaken,the induction rate also would be reduced.[Conclusion] This study will provide the scientific reference in tissue culture and mutation breeding of A.membranaceus Bge.