<正>Objective:To explore the pharmacologic effects of Chinese medicine Bushen Huayu Jiedu Compound Recipe（补肾化瘀解毒复方,BSHYJDR）in drug-resistance cells of lung cancer. Methods:Human lung adenocarcinoma A549/DDP cell strain was selected,serum pharmacology and flow cytometer（FCM）method were adopted,S180 tumor-bearing mice and normal mice were given,through gastrogavage,different doses of a decocted concentration of BSHYJDR.Serum from the abdominal aorta was taken to observe the effect of drug-serum on cisplatin（DDP）concentration, free Ca2+concentration and the expression of lung drug-resistance protein LRP-56 in A549/DDP cells.Results:Compared with the drug-resistance group,the intracellular DDP concentration in the group taking a high dose and the normal group of Chinese medicine showed significant difference （P<0.05）,while no significant difference was found in the low-dose group（P>0.05）.Compared with the drug-resistance group,the Ca2+concentration in cells and the expression of LRP in lung cancer drug-resistance cells A549/DDP of the high-dose group,the low-dose group and the normal group of Chinese medicine were significantly different（all P<0.01）,the LRP expression of the normal group was obviously higher than that of the drug-resistance group（P<0.05）.Conclusion:It was indicated that serum containing Chinese medicine BSHYJDR in the tumor-bearing mice and the normal mice had certainly different,tumor-bearing mice serum containing could improve drug concentration in lung cancer drug-resistance cells,prevent the inflow and release of Ca2+,and inhibit the expression of the drug-resistance gene in the lung cancer drug-resistance cells,which might be the mechanism of BSHYJDR in enhancing the efficacy in reversing and inhibiting tumor.
