树鼩脑缺血后适应升高海马区rCBF及VEGF的变化

被引：8

作者：

罗海芸 ^{[1
]}

李树清 ^{[1
]}

机构：

[1] 昆明医学院病理生理教研室

来源：

基础医学与临床 | 2008年 / 07期

关键词：

光化学; 脑血栓; 缺血后适应; 血管内皮生长因子; 局部脑血流; 树鼩;

D O I：

暂无

中图分类号：

R743.32 [脑血栓形成];

学科分类号：

1002 ;

摘要：

目的探讨缺血后适应(PC)缓解海马rCBF与血管内皮生长因子(VEGF)的变化及其机制。方法建立树鼩血栓性局部脑缺血模型,通过激光多普勒血流计测量海马CA1区rCBF含量;用免疫组化法测定海马VEGF的表达。结果树鼩脑缺血时海马rCBF逐渐降低,以24 h的改变最显著,脑缺血后海马CA1区VEGF阳性细胞数增多,12 h表达最强(P<0.01);缺血PC可显著影响缺血所致的改变:rCBF逐渐增加,72 h最显著(P<0.01),与此同时VEGF的表达除8 h外均比血栓性缺血组增强(P<0.01),12 h组最明显;电镜显示缺血24 h血栓性缺血组的海马线粒体应激及内质网池形成最明显,给予PC后得以缓解。结论缺血12 h内PC通过明显增强VEGF的表达可能与其改善rCBF有关,从而延长治疗的时间窗。

引用

页码：676 / 680

页数：5

共 8 条

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