利用RAPD标记鉴定大豆种质

被引：56

作者：

邱丽娟

RandallL.Nelson

LilaO.Vodkin

机构：

[1] 中国农业科学院作物品种资源研究所

[2] USDASoybeanGermplasmCollection

[3] UrbanaUSA

[4] DepartmentofCropScience

[5] UniversityofIllinois

来源：

作物学报 | 1997年 / 04期

关键词：

PCR; RAPD标记; 大豆;

D O I：

暂无

中图分类号：

S565.1 [大豆];

学科分类号：

摘要：

本研究以57个中国大豆祖先品系及育成品种和18个美国大豆祖先品系为DNA样品来源,通过随机引物PCR扩增基因组DNA的多态性,探索利用RAPD标记鉴定相关种质的可能性。研究结果表明,50个10摩尔随机引物共扩增可分辨产物246个,其中82.4％的随机引物可产生多态性产物,所扩增产物的54.4％至少在两个基因型间存在差异。每个PCR扩增产物分别以1和0记录存在与否。扩增产物间的成对比较可产生非相似性矩阵,此矩阵用于构建遗传相似性的树状系谱图。聚类分析结果表明,中国大豆种质与美国大豆种质不同,两国内的南北方种质也不同。这种明显的地理差异为丰富中国大豆品种遗传基础的种质选择提供了理论依据。

引用

页码：408 / 417

页数：10

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