PRRSV间接免疫荧光检测法的建立和应用

被引：17

作者：

彭长凌

高崧

刘秀梵

机构：

[1] 扬州大学农业部畜禽传染病学重点开放实验室

[2] 扬州大学农业部畜禽传染病学重点开放实验室江苏扬州

[3] 江苏扬州

来源：

中国兽医科技 | 2005年 / 04期

关键词：

猪生殖与呼吸综合征病毒; 单克隆抗体; 间接免疫荧光试验;

D O I：

10.16656/j.issn.1673-4696.2005.04.003

中图分类号：

S852.659.6 [];

学科分类号：

090601 ;

摘要：

以猪生殖与呼吸综合征病毒(PRRSV) ATCC VR 2332 株人工单独感染3 头3 周龄仔猪,并用PRRSV ATCC VR 2332株和猪圆环病毒2型(PCV2)联合感染2 头3 周龄仔猪,每日检查试验猪体温、采食情况及临床表现,并用HerdChek IDEXX 试剂盒测其血清抗体。PRRSV+PCV2联合感染猪分别在感染后1、3周剖杀,PRRSV单独感染猪和健康对照猪分别在感染后1、2和3周剖杀,对全部试验猪的肺组织进行灌洗处理以获得肺泡巨噬细胞(PAM),用PAM进行抹片,同时用肺组织直接进行触片做间接免疫荧光(IIF)检测。结果表明,用所获得的3 株单克隆抗体均检测到人工感染猪肺组织中的PRRSV抗原。另外,用IIF检测7 头临床疑似PRRS病猪的PAM抹片,其中沭阳县的2头猪呈现阳性反应;对这2头猪的肺组织进行病毒分离,在盲传到第4代时Marc 145细胞上产生明显的细胞病变,进一步证实用所获得的3株单克隆抗体检测临床样品的结果准确而可靠,且获得的3株单克隆抗体均可通过IIF用于检测猪肺组织中的PRRSV抗原。

引用

页码：256 / 260

页数：5

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