枫斗类石斛rDNA ITS区的全序列数据库及其序列分析鉴别

被引：56

作者：

丁小余

王峥涛

徐红

徐珞珊

周开亚

不详

机构：

[1] 中国药科大学生药学研究室

[2] 南京师范大学生命科学学院江苏南京

[3] 南京师范大学生命科学学院

[4] 江苏南京

[5] 江苏南京

来源：

药学学报 | 2002年 / 07期

关键词：

石斛属; 枫斗类石斛; rDNAITS区全序列; DNA分子鉴别;

D O I：

10.16438/j.0513-4870.2002.07.016

中图分类号：

R282 [中药材];

学科分类号：

摘要：

目的　建立枫斗类石斛的rDNAITS区碱基全序列数据库 ,利用该数据库对枫斗类石斛待检种进行准确鉴别。方法　对枫斗类石斛的rDNAITS区进行了PCR扩增、测序 ,运用CLUSTRAL ,MEGA等软件以及枫斗类石斛rDNAITS区全序列数据库对待检种rDNAITS区进行序列分析鉴别。结果　建立了 2 1种枫斗类石斛的rDNAITS区全序列数据库 ,枫斗类石斛在该区的种间差异显著而稳定 ,转换和颠换总数为 11～ 12 2 ,变异位点数为 34 1,信息位点数为195。与外类群植物云南石仙桃间的差异较大 ,转换和颠换总数为 131～ 16 1。枫斗类石斛居群间的差异较小 ,转换和颠换总数为 0～ 6。结论　利用枫斗类石斛的全序列数据库及遗传分析软件 ,通过对待检种rDNAITS区进行序列测定 ,可以成功鉴别属于数据库中枫斗类石斛的待检种。

引用

页码：567 / 573

页数：7

共 13 条

[1]

Phylogenetic relationships of 10 grass species: an assessment of phylogenetic utility of the internal transcribed spacer region in nuclear ribosomal DNA in monocots. Hsiao C,Chatterton NJ,Asay KH. Genome . 1994

[2]

rDNA ITS sequencing of herba dendrobii (Huangcao). Xu H,Li XB,Ding XY,et al. Acta Pharm Sin (药学学报) . 2001

[3]

Ribosomal DNA ITS sequences of Cnidium monnieri from different geographical origin in China. Cai JN,Zhou KY,Xu LS,et al. Acta Pharm Sin (药学学报) . 2000

[4]

Morphological and DNA molecular evidence for authentication of Dendrobiumflexicaule from its allied species of Dendrobium. Ding XY,Xu LS,Xu H,et al. Acta Pharm Sin(药学学报) . 2001

[5]

Sequencing of Cyt b gene fragements and PCR identification of "Jinqian Baihuashe"( Bungarus parvus ) and its aduterants. Wang YQ,Zhou KY,Xu LS,et al. Acta Pharm Sin (药学学报) . 1998

[6]

Molecular phylogenetics of Diseae (Orchidaceae): a contribution from nuclear ribosomal ITS sequence. Douzery EJP,Pridgeon AM,Kores P,et al. American Journal of Botany . 1999

[7]

Characters of nrDNA ITS region sequences of fruits of Alpinia galanga and their adulterants. Zhao ZL,Zhou KY,Dong H,et al. Planta Medica . 2001

[8]

A new approach for the identification of a Chinese traditional medicine"Chuanxiong"by 18S ribosomal RNA gene sequences. Fushimi H,Komatsu K,Isobe M. Phytomedicine . 1996

[9]

Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: an example from the Compositae. Baldwin BG. Molecular Phylogenetics and Evolution . 1992

[10]

Studies on rDNA ITS1 regions of soybean and its wild relatives. Gu J,Hui DW,Zhuang BC,et al. Acta Bot Sin(植物学报) . 1994

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