枫斗类石斛rDNA ITS区的全序列数据库及其序列分析鉴别

被引:56
作者
丁小余
王峥涛
徐红
徐珞珊
周开亚
不详
机构
[1] 中国药科大学生药学研究室
[2] 南京师范大学生命科学学院 江苏南京
[3] 南京师范大学生命科学学院
[4] 江苏南京
[5] 江苏南京
关键词
石斛属; 枫斗类石斛; rDNAITS区全序列; DNA分子鉴别;
D O I
10.16438/j.0513-4870.2002.07.016
中图分类号
R282 [中药材];
学科分类号
摘要
目的 建立枫斗类石斛的rDNAITS区碱基全序列数据库 ,利用该数据库对枫斗类石斛待检种进行准确鉴别。方法 对枫斗类石斛的rDNAITS区进行了PCR扩增、测序 ,运用CLUSTRAL ,MEGA等软件以及枫斗类石斛rDNAITS区全序列数据库对待检种rDNAITS区进行序列分析鉴别。结果 建立了 2 1种枫斗类石斛的rDNAITS区全序列数据库 ,枫斗类石斛在该区的种间差异显著而稳定 ,转换和颠换总数为 11~ 12 2 ,变异位点数为 34 1,信息位点数为195。与外类群植物云南石仙桃间的差异较大 ,转换和颠换总数为 131~ 16 1。枫斗类石斛居群间的差异较小 ,转换和颠换总数为 0~ 6。结论 利用枫斗类石斛的全序列数据库及遗传分析软件 ,通过对待检种rDNAITS区进行序列测定 ,可以成功鉴别属于数据库中枫斗类石斛的待检种。
引用
收藏
页码:567 / 573
页数:7
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