重症手足口病患儿血清细胞因子检测分析

被引:26
作者
王爽
赵艳
张欣
张海萍
冯霞
闫惠平
机构
[1] 首都医科大学附属北京佑安医院感染与免疫研究中心
关键词
细胞因子; 手足口病; 液相芯片技术;
D O I
10.15932/j.0253-9713.2012.03.026
中图分类号
R725.1 [小儿传染病];
学科分类号
摘要
目的探讨重症手足口病(HFMD)患儿血清多种细胞因子的变化水平,了解患儿的免疫功能状态。方法重症HFMD患儿34例,根据病情分为重型组和危重型2组;选择16例健康儿童作为对照组。总结HFMD患儿的临床特点,进行病原学检测,同时采用Luminex液相芯片方法检测3组患儿血清中的8种细胞因子,包括白细胞介素(IL)-2、IL-4、IL-6、IL-8、IL-10、粒细胞/巨噬细胞集落刺激因子(GM-CSF)、肿瘤坏死因子-α(TNF-α)和干扰素-γ(IFN-γ)。结果重型组25例,危重型组9例。与对照组比较,重型组IL-2和IL-10明显升高(P<0.05);除IL-8外,危重型组中的7种细胞因子与重型组和对照组比较,均显著升高(P<0.005,P<0.001);脑炎合并肺炎组(13例)与脑炎组(17例)比较,IL-6显著升高(P<0.05);EV71阳性组(8例)与病原学未检出组(16例)比较,IL-2、IL-4、IL-6、IL-10、GM-CSF、IFN-γ和TNF-α明显升高(P<0.05)。结论多种细胞因子参与了HFMD重症病例的发病和发展,产生严重的炎症和抗炎反应。EV71阳性病例具有更加明显的免疫反应。
引用
收藏
页码:185 / 188
页数:4
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