Vaspin对HUVECs中TNF-α介导的NF-κB和PI3K/Akt信号通路的影响

被引：4

作者：

刘师伟 ^{[1
]}

王明明 ^{[2
]}

王军 ^{[2
]}

楼晓华 ^{[3
]}

董艳婷 ^{[2
]}

张丽 ^{[2
]}

赵术君 ^{[2
]}

何玉洁 ^{[4
]}

机构：

[1] 山西医科大学附属大医院内分泌科

[2] 山西医科大学研究生学院

[3] 休斯敦Methodist代谢研究中心

[4] 山西医科大学第一医院妇产科

来源：

基础医学与临床 | 2015年 / 35卷 / 08期

关键词：

Vaspin; 核因子-κB; 磷脂酰肌醇3激酶(PI3K)/Akt; 胰岛素抵抗; 炎性反应; 血管内皮细胞;

D O I：

10.16352/j.issn.1001-6325.2015.08.003

中图分类号：

R58 [内分泌腺疾病及代谢病];

学科分类号：

摘要：

目的探讨Vaspin对TNF-α介导的人脐静脉内皮细胞(HUVECs)NF-κB和PI3K/Akt信号通路的影响。方法体外分离并培养HUVECs。NF-κB荧光酶报告质粒瞬时转染HUVECs,用不同浓度(0～320μg/L)Vaspin预培养,随后用10μg/L的TNF-α作用于HUVECs,荧光酶报告分析法测定NF-κB的转录活性。Western blot测定磷酸化的Akt水平。ELISA测定细胞上清液中IL-1及IL-6的浓度。Real-time PCR、Western blot检测细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)及单核细胞趋化蛋白-1(MCP-1)的mRNA和蛋白表达水平。结果在HUVECs中,Vaspin抑制TNF-α介导的NF-κB转录活性(P<0.05),并且NF-κB下游因子IL-1、IL-6、ICAM-1、VCAM-1和MCP-1的表达降低(P<0.05)。Vaspin增加了炎性因子TNF-α介导的磷酸化的Akt水平(P<0.05)。结论 Vaspin抑制HUVECs中TNF-α介导的NF-κB信号通路,增强TNF-α介导的PI3K/Akt信号通路的传导。

引用

页码：1020 / 1024

页数：5

共 7 条

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