西红花酸对自由基发生系统诱导DNA损伤的保护作用(英文)

被引：7

作者：

刘同征

陆宇

包晨颖

钱之玉

机构：

[1] 中国药科大学药理学教研室

[2] 中国药科大学药理学教研室南京

[3] 南京

来源：

中国药科大学学报 | 2002年 / 06期

关键词：

西红花酸; OH·; Vc; 过氧化氢; DNA碱基; 损伤; 保护; 自由基发生系统;

D O I：

暂无

中图分类号：

R965 [实验药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的　研究西红花酸对不同自由基发生系统诱导的 DNA损伤的保护作用。方法　通过葡聚糖凝胶、TBA反应、紫外碱基吸收来检测西红花酸对 DNA的保护作用。结果　 DNA被 OH·进攻损伤后 ,碱基在 2 60 nm处紫外吸收下降 ,DNA脱氧核糖氧化破坏 ,其氧化产物与 TBA反应在 5 3 2 nm处出现特征吸收 ,DNA的链结构破坏 ,痕量的金属离子可以加速 DNA的损伤。西红花酸可以显著抑制 DNA的上述损伤。结论　西红花酸具有很强的抗自由基作用 ,对 DNA具有保护作用

引用

页码：51 / 55

页数：5

共 9 条

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