转SCaM-GFP融合基因烟草中钙调素分泌特性的研究

被引：4

作者：

周华林

马力耕

刘曼

毛国红

孙大业

机构：

[1] 河北师范大学分子细胞生物学实验室,河北师范大学分子细胞生物学实验室,河北师范大学分子细胞生物学实验室,河北师范大学分子细胞生物学实验室,河北师范大学分子细胞生物学实验室石家庄,石家庄,石家庄,石家庄,石家庄

来源：

植物学报 | 2001年 / 12期

基金：

国家杰出青年科学基金; 国家自然科学基金重点项目;

关键词：

大豆钙调素亚型; 绿色荧光蛋白; 分泌;

D O I：

暂无

中图分类号：

Q945 [植物生理学];

学科分类号：

0903 ;

摘要：

The binary vectors which respectively contain chimeric genes (SCaM1-GFP, SCaM4-GFP) were constructed and used to transform tobacco (Nicotiana tabacumL.), pGTV-GFP was used as control. The plasmolyzed cells of transgenic callus treated with CBW were investigated under the laser scanning confocal microscope. Green fluorescence was found in the cell wall of transgenic SCaM1-GFP callus. However there was no green fluorescence in the cell wall of SCaM4-GFP or GFP transgenic callus. These results indicate that SCaM1 can be secreted into the apoplast of plant cells, while SCaM4 does not exist in the apoplast of plant cells.

引用

页码：1300 / 1302

页数：3