大鼠肺泡巨噬细胞吞噬煤尘颗粒的实验研究

被引:4
作者
遇铭
万恩新
顾国强
雷伟宏
高俊玲
瓦龙美
机构
[1] 华北煤炭医学院组织学胚胎学教研室
关键词
吞噬作用; 吖啶橙; 煤尘; 肺泡巨噬细胞; 荧光显微镜; 透射电镜;
D O I
10.16098/j.issn.0529-1356.1988.02.028
中图分类号
学科分类号
摘要
本实验用相差显微电影、溶酶体荧光定位和透射电镜等方法,研究了大鼠离体肺泡巨噬细胞吞噬煤尘的作用,并用二氧化矽和酵母菌作实验对照。结果表明,煤尘颗粒同二氧化矽类似,经过附着,迅速以吞噬体形式被摄入胞浆,而酵母菌呈典型的伪足包围和缓幔吞噬过程。实验证明,用吖啶橙作活细胞溶酶体定位是有效的。被标记的溶酶体发出强橙色荧光。当煤粒吞噬体进入胞浆后,常被多个溶酶体接触包围,形成次级溶酶体。电镜观察提示,煤尘可引起次级溶酶体膜的损害。
引用
收藏
页码:206 / 209+240 +240
页数:5
相关论文
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