裂褶菌产纤维二糖脱氢酶条件优化及部分酶学性质研究

被引:9
作者
方靖
刘稳
高培基
机构
[1] 山东大学微生物技术国家重点实验室!济南250100
关键词
裂褶菌; 纤维二糖脱氢酶; 酶的合成; 酶性质研究;
D O I
10.13346/j.mycosystema.2000.01.022
中图分类号
Q939.97 [工业微生物学];
学科分类号
082203 ;
摘要
研究了裂褶菌Schizophyllum communeAS 5.391产纤维二糖脱氢酶的条件。该酶是诱导酶,棉花是最好的诱导底物。培养基中加入琥珀酸钠缓冲液和土温80利于酶的合成。而木素相关物质黎芦醇或愈创木酚对酶的生成没有影响。该酶在pH3.5~10.5和45℃以下保持稳定,其最适作用温度为30℃,最适pH为45。Zn(2+)和Ag+对该酶活性有很大的抑制作用,而叠氮化钠和氰化钾对酶活力没有影响。
引用
收藏
页码:107 / 110
页数:4
相关论文
共 7 条
[1]  
Conlinuous monitoring of cellulose oxidation by cellobiose oxidase from Phanerochaete chrysosporium. Kremer S M,Wood P M. FEMS Microbiology Letters . 1992
[2]  
Recent advances in fungal cellobiose oxidoreductases. K. E. L. Eriksson,N. Habu,M. Samejima. Enzyme and Microbial Technology . 1993
[3]  
Kraft pulp bleaching and delignification by Trametes versicolor J. FS Archibald,R Bourbonnais,L Jurasek,MG Paice,ID Reid. Journal of Biotechnology . 1997
[4]   Wide distribution of cellobiose-oxidizing enzymes in wood-rot fungus indicates a physiological importance in lignocellulosics degradation [J].
Fang, J ;
Qu, YB ;
Gao, PJ .
BIOTECHNOLOGY TECHNIQUES, 1997, 11 (03) :195-197
[5]  
Wide distribution of cellobiose-oxidizing enzymes in wood-rot fungus indicates a physiological importance in lignocellulosics degradation. Fang J,et al. Biotechnology Techniques . 1997
[6]  
Production of Fenton’s reagent by cellobiose oxidase from cellulolytic cultures of Phanerochaete chrysosporium. Rremer K.M,Wood P M. European Journal of Biochemistry . 1992
[7]  
The cellobiose-oxidizing enzymes CBQ and CBO as related to lignin and cellulose degradation-areview. Ander P. FEMS Microbiology Reviews . 1994