限食对D-半乳糖脑老化小鼠大脑抗自由基酶活力的保护作用

被引：4

作者：

张淑华

肖忠新

褚金花

吴惠慧

魏守刚

机构：

[1] 首都医科大公共卫生与家庭医学学院

来源：

毒理学杂志 | 2010年 / 24卷 / 01期

基金：

北京市自然科学基金;

关键词：

脑老化; 限食; SOD; GSH-Px; D-半乳糖;

D O I：

10.16421/j.cnki.1002-3127.2010.01.015

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的探讨限食对D-半乳糖诱导的脑老化小鼠脑组织氧化损伤的保护作用。方法3月龄ICR小鼠40只分4组,即脑老化模型组、脑老化限食组、限食组和普通对照组。以D-半乳糖100mg/(kg·d)皮下注射法建立脑老化模型,限食鼠则按正常热能摄入量的70%投喂。实验干预9周后,先以Morris水迷宫实验测定小鼠空间学习记忆能力,再取大脑测定抗氧化酶SOD、GSH-Px活性及丙二醛(MDA)含量。结果①水迷宫实验:D-半乳糖脑老化小鼠逃避潜伏期明显大于对照小鼠(P<0.05),限食处理的脑老化小鼠逃避潜伏期显著低于脑老化鼠(P<0.05)而与正常对照组无显著差异。②生化检测:D-半乳糖脑老化小鼠脑内SOD、GSH-Px活性显著低于对照小鼠(P<0.05),限食脑老化小鼠脑内SOD、GSH-Px活性显著高于脑老化鼠(P<0.05)而与正常对照组无显著差异。但限食对正常小鼠脑组织SOD、GSH-Px活性未见显著影响。脑老化小鼠脑内MDA含量显著升高而限食可使MDA含量维持在接近正常对照组水平。结论在本试验条件下,限食能减轻脑老化小鼠认知功能衰退及脑组织抗超氧阴离子自由基酶活性下降,保护大脑免遭D-半乳糖氧化损伤所致脑老化。

引用

页码：55 / 58

页数：4

共 8 条

[1] Long-term D-galactose injection combined with ovariectomy serves as a new rodent model for Alzheimer's disease [J].

Hua, Xiangdong ;

Lei, Ming ;

Zhang, Yongjie ;

Ding, Jiong ;

Han, Qunying ;

Hu, Gang ;

Xiao, Ming .

LIFE SCIENCES, 2007, 80 (20) :1897-1905

[2] D-Galactose injured neurogenesis in the hippocampus of adult mice [J].

Zhang, Q ;

Li, XK ;

Cui, X ;

Zuo, PP .

NEUROLOGICAL RESEARCH, 2005, 27 (05) :552-556

[3]

Dietary restriction and 2‐deoxyglucose administration reduce focal ischemic brain damage and improve behavioral outcome: Evidence for a preconditioning mechanism[J] . Zai FangYu,Mark P.Mattson.J. Neurosci. Res. . 1999 (6)

[4] Molecular biology of aging [J].

Johnson, FB ;

Sinclair, DA ;

Guarente, L .

CELL, 1999, 96 (02) :291-302

[5]

The Endoplasmic Reticulum Stress-Responsive Protein GRP78 Protects Neurons Against Excitotoxicity and Apoptosis: Suppression of Oxidative Stress and Stabilization of Calcium Homeostasis[J] . ZaiFang Yu,Hong Luo,Weiming Fu,Mark P. Mattson.Experimental Neurology . 1999 (2)

[6] SOD and catalase inactivation by singlet oxygen and peroxyl radicals [J].

Escobar, JA ;

Rubio, MA ;

Lissi, EA .

FREE RADICAL BIOLOGY AND MEDICINE, 1996, 20 (03) :285-290

[7] EFFECTS OF DIETARY RESTRICTION ON MOTOR LEARNING AND CEREBELLAR NORADRENERGIC DYSFUNCTION IN AGED F344 RATS [J].

GOULD, TJ ;

BOWENKAMP, KE ;

LARSON, G ;

ZAHNISER, NR ;

BICKFORD, PC .

BRAIN RESEARCH, 1995, 684 (02) :150-158

[8]

Effects of Geno-type and Diet on Age-related Lesions in ad LibitumFed and Calorie-restricted F-344,BN,and BNF3F1Rats. Lipman RD,Dallal GE,Bronson RT. Journal of Gerontology . 1999

← 1 →