微载体悬浮培养成人骨髓间充质干细胞

被引：18

作者：

吴清法

吴祖泽

董波

王立生

机构：

[1] 北京放射医学研究所,北京放射医学研究所,北京放射医学研究所,北京放射医学研究所北京,军事医学科学院生物工程研究所工作,北京,北京,北京,北京

来源：

中国实验血液学杂志 | 2003年 / 01期

关键词：

间充质干细胞; 细胞培养; 微载体; 组织工程;

D O I：

暂无

中图分类号：

R329 [人体组织学];

学科分类号：

100101 ;

摘要：

本研究采用微载体旋转培养系统和常规静止培养系统对成人骨髓间充质干细胞 (mesenchymalstemcell,MSC)的培养进行比较。MSC是贴壁依赖性细胞 ,旋转培养系统采用CultiSpherG大孔微载体 ,浓度为 1g L ,常规静止培养在 12孔培养板中进行。两系统细胞接种密度均为 5× 10 4 cells ml。结果 :旋转培养 7天后达到最大活细胞密度5 .15 0× 10 5cells ml,常规静止培养第 5天就达到最大活细胞密度 1.6 75× 10 5cells ml。在微载体旋转培养中生成乳酸12 .0 6mmol L ,而常规静止培养中生成乳酸 13.10mmol L ,葡萄糖消耗分别为 7.38mmol L和 5 .37mmol L。在微载体旋转培养中平均乳酸产率为 1.6 3,远低于常规静止培养的平均乳酸产率 2 .4 4。这些表明 ,在微载体悬浮培养条件下 ,MSC生长更为旺盛 ,细胞产量更高 ,葡萄糖消耗和能量利用率优于常规静止培养。微载体悬浮培养 12天后 ,MSC依然保持其干细胞特性。结论 :微载体培养系统是扩增组织工程种子细胞的有效方法

引用

页码：15 / 21

页数：7

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