硫化砷诱导K562细胞凋亡中Bcl-2和Bax的表达

被引：15

作者：

陈思宇

刘陕西

李信民

机构：

[1] 西安交通大学第一医院

[2] 西安交通大学第一医院陕西西安

[3] 陕西西安

来源：

第四军医大学学报 | 2002年 / 09期

关键词：

Bcl-2; 砷化物; 凋亡; 雄黄; 白血病,髓样,慢性;

D O I：

暂无

中图分类号：

R73-36 [治疗实验];

学科分类号：

100214 ;

摘要：

目的　研究 Bcl- 2和 Bax在硫化砷诱导的 K5 6 2细胞凋亡中的表达 ,探讨其在硫化砷诱导的细胞凋亡中的作用 .方法　 K5 6 2细胞经一定剂量硫化砷作用特定时间后 ,MTT法检测细胞生长、增殖状态 ,光镜及透射电镜下观察细胞形态及凋亡情况 ,S- P免疫组化染色检测 Bcl- 2、Bax的表达并进行图像分析 .结果　硫化砷对 K5 6 2细胞生长增殖有时间剂量依赖性抑制作用 ;175～ 14 0 0μg· L- 1 硫化砷作用 18～ 14 4 h后 ,K5 6 2细胞出现凋亡细胞的形态学改变 ;免疫组化染色表明 K5 6 2细胞低表达 Bcl- 2蛋白 (A值为 0 .15 2± 0 .0 2 3,阴性对照 A值 0 .10 8± 0 .0 12 ) ,硫化砷处理后对其表达无明显影响 (A值为 0 .138± 0 .0 2 8,P>0 .0 5 ) .硫化砷作用后 ,Bax蛋白的表达量 A值由 0 .2 4 0± 0 .0 13增加到 0 .316± 0 .0 2 1(P<0 .0 1) .结论　硫化砷可抑制 K5 6 2细胞的生长和增殖并诱导其发生凋亡 ,其机制可能与 Bax蛋白上调有关 .

引用

页码：790 / 792

页数：3

共 14 条

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