紫杉醇载药胶束对人肺癌A549细胞增殖与凋亡的影响

被引：6

作者：

王琳 ^{[1
]}

余瑞双 ^{[1
]}

杨文亮 ^{[1
]}

栾淑娟 ^{[1
]}

秦本凯 ^{[1
]}

庞晓斌 ^{[1
]}

杜冠华 ^{[2
]}

机构：

[1] 河南大学药物研究所

[2] 中国医学科学院北京协和医学院药物研究所国家药物筛选中心

来源：

药学学报 | 2015年 / 10期

关键词：

紫杉醇; 胶束; 肺癌A549细胞; 增殖; 细胞周期; 凋亡;

D O I：

暂无

中图分类号：

R734.2 [肺肿瘤];

学科分类号：

100117 [系统生物医学];

摘要：

本文研究了以聚乙二醇-聚己内酯共聚物为载体制备的紫杉醇载药胶束(m PEG-PCL-PTX)对人肺癌A549细胞增殖和凋亡的影响,探讨其抗肿瘤的可能机制。以体外培养的人肺癌A549细胞为研究对象,四甲基偶氮唑盐(MTT)法检测m PEG-PCL-PTX对细胞增殖的抑制作用;流式细胞仪检测细胞周期;Hoechst/PI染色检测细胞凋亡;RT-PCR检测A549细胞Bax、Bcl-2、Caspase-3 m RNA的表达;Western blot检测A549细胞Bax、Bcl-2蛋白表达。结果显示,空白聚合物胶束体外基本无毒性,m PEG-PCL-PTX对A549细胞增殖有明显抑制作用,并使细胞周期阻滞于G2/M期;载药胶束促进A549细胞凋亡,使A549细胞Bcl-2基因和蛋白表达下调,Bax表达上调,同时上调Caspase-3基因表达。结果表明,含腙键p H敏感聚合物体外基本无毒性,载药胶束m PEG-PCLPTX对人肺癌A549细胞的增殖有抑制作用,对A549细胞的凋亡有促进作用。

引用

页码：1240 / 1245

页数：6

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