电针预处理对MCAO大鼠模型血脑屏障和MMP-9的影响

被引：10

作者：

邢东 ^{[1
]}

吴志新 ^{[1
]}

董辉 ^{[1
]}

范延红 ^{[2
]}

机构：

[1] 第四军医大学西京医院麻醉科

[2] 第四军医大学西京医院心脏内科

来源：

神经解剖学杂志 | 2017年 / 33卷 / 04期

关键词：

电针预处理; 缺血再灌注损伤; 基质金属蛋白酶-9; 血脑屏障; 紧密连接蛋白; 脑水肿; 大鼠;

D O I：

10.16557/j.cnki.1000-7547.2017.04.003

中图分类号：

R245 [针灸学、针灸疗法]; R-332 [医用实验动物学];

学科分类号：

100512 ;

摘要：

目的:探讨电针(EA)预处理对大鼠脑缺血再灌注后血脑屏障(BBB)通透性及脑内基质金属蛋白酶-9(MMP-9)表达的影响。方法:96只成年雄性SD大鼠随机分为假手术(Sham)组、单纯电针(EA)组、缺血(MCAO)组及电针预处理(EA+MCAO)组,每组24只。使用线栓法制作大鼠大脑中动脉阻塞(MCAO)模型,缺血120 min再灌注48 h后处死取脑。分别采用干湿重法测定各组动物脑水含量,伊文斯蓝(EB)法检测血脑屏障通透性,Western Blot检测紧密连接蛋白(TJP)及MMP-9表达水平,同时明胶酶谱法检测MMP-9活性。结果:与Sham组相比,MCAO组大鼠脑水含量及EB含量增多(P<0.05),缺血侧脑组织TJP水平下降(P<0.05),MMP-9表达及活性明显升高(P<0.05);与MCAO组大鼠比较,EA+MCAO组脑水含量及EB含量减少(P<0.05),缺血侧脑组织TJP水平升高(P<0.05),同时MMP-9表达及活性显著降低(P<0.05)。结论:电针预处理通过抑制缺血后脑内MMP-9的表达及活性,维持BBB完整性,有效改善脑缺血后的脑水肿症状。

引用

页码：383 / 389

页数：7

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