补阳还五汤对去势脑缺血雌性大鼠海马神经干细胞增殖及ERK/CREB信号通路的影响

被引：11

作者：

黄昕 ^{[1
]}

周胜强 ^{[2
]}

刘胜贤 ^{[1
]}

张必超 ^{[1
]}

薛科辉 ^{[1
]}

刘柏炎 ^{[1
,2
]}

机构：

[1] 益阳医学高等专科学校附属医院

[2] 湖南中医药大学

来源：

湖南中医药大学学报 | 2016年 / 36卷 / 12期

关键词：

脑缺血; 神经干细胞; ERK/CREB信号通路; 去势大鼠; 补阳还五汤;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的探讨补阳还五汤对去势脑缺血雌性大鼠海马神经干细胞(neural stem cells,NSCs)增殖及ERK/CREB信号通路的影响。方法采用双侧卵巢切除术(OVX)结合大脑中动脉阻塞(MCAO)法复制去势雌性大鼠脑缺血复合模型,将造模后的36只大鼠随机分为双假手术组、复合模型组、雌激素组、雌激素+G15组、补阳还五汤组及补阳还五汤+G15组6组,每组6只,术后24 h给予相应药物灌胃连续干预14 d,同时每天腹腔注射Brd U、G15分别标记增殖细胞和阻断GPER-1。采用免疫荧光Brd U/Nestin双标法检测各组大鼠缺血侧海马齿状回NSCs增殖情况,免疫组化SP法检测ERK1/2、CREB1磷酸化蛋白表达水平。结果补阳还五汤组和雌激素组缺血侧海马DG区Brd U/Nestin双标阳性细胞及p ERK1/2、p CREB1阳性细胞表达均增加,与复合模型组比较有显著性差异(P<0.05),但补阳还五汤组要多于雌激素组(P<0.05)。与补阳还五汤组比较,补阳还五汤+G15组缺血侧海马DG区Brd U/Nestin双标阳性细胞及及p ERK1/2、p CREB1阳性细胞表达均减少(P<0.05)。结论补阳还五汤可以促进去势脑缺血雌性大鼠缺血侧海马DG区神经干细胞增殖,激活ERK/CREB信号通路,可能是其发挥类雌激素作用、促进内源性神经再生作用机制之一。

引用

页码：1 / 6

页数：6

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