顺式-和反式-阿霍烯在Caco-2细胞模型中的体外摄取、转运和外排特性

被引：5

作者：

田莉

杨秀伟

王莹

徐嵬

机构：

[1] 北京大学药学院天然药物及仿生药物国家重点实验室

来源：

药学学报 | 2007年 / 01期

基金：

国家高技术研究发展计划(863计划);

关键词：

阿霍烯; 大蒜; Caco-2细胞单层; 跨膜转运;

D O I：

10.16438/j.0513-4870.2007.01.016

中图分类号：

R285 [中药药理学];

学科分类号：

摘要：

研究顺式-阿霍烯(Z-Ajo)和反式-阿霍烯(E-Ajo)的肠细胞摄取、转运和外排特性。采用体外培养的人结肠Caco-2细胞单层模型评价,应用高效液相色谱法测定Z-Ajo和E-Ajo的含量。结果表明,仅能在Caco-2细胞单层的顶侧检测到Z-Ajo或E-Ajo;阿霍烯在Caco-2细胞中的代谢可被抗氧化剂维生素C、细胞色素P450药物代谢酶3A亚型抑制剂甲吡酮和ATP抑制剂叠氮化钠所抑制。Z-Ajo和E-Ajo皆不能透过Caco-2单层细胞而被迅速代谢,其代谢与CYP450药物代谢酶有关。

引用

页码：87 / 92

页数：6

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