大鼠骨髓间充质干细胞分离鉴定方法及生物学特性

被引：40

作者：

曹华 ^{[1
]}

高建华 ^{[1
]}

刘小龙 ^{[1
]}

林思思 ^{[2
]}

机构：

[1] 江西医学高等专科学校

[2] 南昌大学医学部

来源：

中国组织工程研究 | 2017年 / 21卷 / 09期

关键词：

骨髓; 间质干细胞; 细胞增殖; 组织工程; 干细胞; 骨髓干细胞; 骨髓间充质干细胞; 全骨髓贴壁培养; 生物学特性; 流式细胞仪; 标记物; 增殖能力;

D O I：

暂无

中图分类号：

R318.08 [生物材料学]; R68 [骨科学（运动系疾病、矫形外科学）];

学科分类号：

100103 [病原生物学]; 100220 [骨科学];

摘要：

背景:如何能简便高效地获得均质性的骨髓间充质干细胞群是软骨组织工程研究的重点。目的:分离培养大鼠骨髓间充质干细胞,观察其生物学特性。方法:抽取30只SD大鼠股骨以及胫骨骨髓,采用全骨髓贴壁培养法体外分离培养骨髓间充质干细胞,流式细胞仪测定细胞表面标记物的表达,细胞计数法观察细胞生长增殖能力,MTT法检测细胞存活率。结果与结论:(1)刚分离培养的细胞呈圆形、椭圆形,部分细胞呈三角形,接种12-15 d后,细胞开始融合,传代后细胞增殖速度加快,传代2 h后细胞均匀分布在瓶底;(2)随着传代次数的增加,细胞增殖能力出现下降趋势,第1-5代细胞存活率均超过95%,显著高于第6代和第7代(P<0.05);(3)骨髓间充质干细胞表面CD34呈阴性表达,CD44呈阳性表达;(4)结果表明,采用全骨髓贴壁法可有效分离大鼠骨髓间充质干细胞,获得的细胞生长稳定,增殖能力强,可选用前5代细胞作为软骨组织工程的种子细胞。

引用

页码：1357 / 1361

页数：5

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