代谢性酸中毒诱导新生大鼠视网膜新生血管的实验研究

被引：2

作者：

陈宜

黎晓新

Jonathan M.Holmes

机构：

[1] 北京大学人民医院眼科中心

[2] 美国纽约医学中心眼科

来源：

中华眼底病杂志 | 2005年 / 05期

关键词：

视网膜病,早产儿/病因学; 酸中毒; 内皮生长因子; 视网膜新生血管; 疾病模型,动物;

D O I：

暂无

中图分类号：

R774.1 [视网膜疾病];

学科分类号：

100212 ;

摘要：

目的了解代谢性酸中毒诱导新生大鼠视网膜病变的发展进程,探讨其发生与血管内皮生长因子(VEGF)的关系。方法实验组新生Sprague-Dawley大鼠425只。从大鼠出生后第2天开始按535mg/kg的剂量管饲NH4Cl(浓度为50mg/ml),每天2次,管饲6d,然后进入恢复期。另选150只新生大鼠未进行管饲,作为对照组。两组大鼠分别于出生后3、5、8、10、13、20d处死。取出双眼进行视网膜铺片和二磷酸腺苷酶染色,对视网膜血管进行评估;用酶联免疫分析法进行VEGF的测定。结果实验组鼠在出生后3、5、8、10、13、20d的新生血管(NV)发生率分别为0%、9%、26%、55%、19%、0%。出生后第3天,实验组鼠VEGF的蛋白水平[(101.1±14.2)pg/mg]比对照组[(133.2±15.9)pg/mg]下降(P=0.004),出后生第8天,实验组鼠VEGF蛋白水平[(98.4±19.2)pg/mg]比对照组[(78.1±8.7)pg/mg]升高(P=0.028);出生后第5、10、13、20天,两组差异无统计学意义(P>0.05)。结论代谢性酸中毒可能损害了正在发育的视网膜血管而引起新生血管;酸中毒引起的视网膜新生血管与VEGF有关。

引用

页码：296 / 299

页数：4

共 12 条

[1]

Multicenter trial of cryotherapy for retinopathy of prematuity.Snellen visual acuity and structural outcome at 5 and 6 months years after randomization. Crytherapy for Retinopathy of Prematurity Cooperative Group. Archives of Ophthalmology . 1996

[2]

Vascular endothelial growth factor vascular permeability factor expression in a mouse model of retinal neovascularization. Pierce EA,Avery RL,Foley ED,et al. Proceedings of the National Academy of Sciences of the United States of America . 1995

[3]

Metabolic acidosisinduced retinopathy in the neonatal rat. Holmes JM,Zhang S,Leske DA,et al. Investigative Ophthalmology . 1999

[4]

Carbon dioxide-induced retinopathy in the neonatal rat. Holmes JM,Zhang S,Leske DA,et al. Current Eye Research . 1998

[5]

Preretinal neovascularization in the neonatal rat associated with acetazolamide-induced systemic acidosis. Zhang S,Leske DA,Lanier WL,et al. Investigative Ophthalmology . 2001

[6]

Vascular endothelial growth factor acts as a survival factor for newly formed retinal vessels and has implications for retinopathy of prematurity. Alon T,Hemo I,Itin A,et al. Nature Medicine . 1995

[7]

Correlation of vascular permeability factor vascular endothelial growth factor with extraretinal neovascularization in the rat. Dorey CK,Aouididi S,Reynaud X,et al. Archives of Ophthalmology . 1996

[8]

Clinical factors associated with retinopathy of prematurity. Prederville A,Suchulenburg WE. Archives of Disease in Childhood . 1988

[9]

A new technique for visualization of human retinal vasculature. Lutty GA,McLeod DS. Archives of Ophthalmology . 1992

[10]

Neovascularization grading methods in a rat model of retinopathy of prematurity. Zhang S,Leske DA,Holmes JM. Investigative Ophthalmology . 2000

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