实时荧光定量PCR(TaqMan)法测定外源基因的拷贝数

被引：50

作者：

王爱民 ^{[1
,2
]}

机构：

[1] 徐州师范大学生命科学学院

[2] 江苏省药用植物生物技术重点实验室

来源：

广西植物 | 2009年 / 29卷 / 03期

关键词：

外源基因; 拷贝数; 实时荧光定量PCR;

D O I：

暂无

中图分类号：

Q75 [分子遗传学];

学科分类号：

071007 [遗传学];

摘要：

实时荧光定量PCR是近年新兴的一项技术,因其快速、方便、便宜,需要DNA样品量少,无需放射性检测等优点被广泛应用于基因的定量分析。该文就实时荧光定量PCR(TaqMan)技术的发展、基本原理及测定外源基因拷贝数的技术流程做一介绍。

引用

页码：408 / 412

页数：5

共 8 条

[1]

Assessment of transgenic maize events produced by particle bombardment or <Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated transformation.[J].Huixia Shou;Bronwyn R. Frame;Steven A. Whitham;Kan Wang.Molecular Breeding.2004, 2

[2]

Estimating number of transgene copies in transgenic rapeseed by real-time PCR assay with HMG I/Y as an endogenous reference gene [J].

Weng, H ;

Pan, AH ;

Yang, LT ;

Zhang, CM ;

Liu, ZL ;

Zhang, DB .

PLANT MOLECULAR BIOLOGY REPORTER, 2004, 22 (03) :289-300

[3]

Comparison of two quantitative polymerase chain reaction methods for detecting HER2/neu amplification [J].

Millson, A ;

Suli, A ;

Hartung, L ;

Kunitake, S ;

Bennett, A ;

Nordberg, MCL ;

Hanna, W ;

Wittwer, CT ;

Seth, A ;

Lyon, E .

JOURNAL OF MOLECULAR DIAGNOSTICS, 2003, 5 (03) :184-190

[4]

Quantitative real-time PCR assay for determining transgene copy number in transformed plants [J].

Ingham, DJ ;

Beer, S ;

Money, S ;

Hansen, G .

BIOTECHNIQUES, 2001, 31 (01) :132-+

[5]

Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].

Livak, KJ ;

Schmittgen, TD .

METHODS, 2001, 25 (04) :402-408

[6]

Quantitative Reverse Transcription–Polymerase Chain Reaction to Study mRNA Decay: Comparison of Endpoint and Real-Time Methods.[J].Thomas D. Schmittgen;Brian A. Zakrajsek;Alan G. Mills;Vladimir Gorn;Michael J. Singer;Michael W. Reed.Analytical Biochemistry.2000, 2

[7]

Identification of Leptospira biflexa by real-time homogeneous detection of rapid cycle PCR product [J].