热带水果多酚提取物的抗氧化和抗增殖活性研究

被引：21

作者：

李武 ^{[1
]}

李艳君 ^{[2
]}

杨瑞丽 ^{[2
]}

机构：

[1] 海南大学食品学院

[2] 华南农业大学食品学院

来源：

现代食品科技 | 2013年 / 29卷 / 10期

基金：

海南省自然科学基金;

关键词：

热带水果; 多酚; 抗氧化; 抗增殖;

D O I：

10.13982/j.mfst.1673-9078.2013.10.007

中图分类号：

TS201.2 [食品化学]; R285 [中药药理学];

学科分类号：

100403 [营养与食品卫生学]; 100806 [中药药理学];

摘要：

为明确12种热带水果多酚提取物的总酚含量、总抗氧化能力和抗肿瘤细胞增殖活性,分别采用Folin-Ciocalteu法确定了水果提取物的总酚含量,采用ORAC和FRAP的方法确定了其抗氧化能力,采用MTT的方法确定了其抗人肝癌细胞HepG2增殖的活性。结果显示,12种水果提取物的总酚含量为26.17～229.67 mg GAE/100 g鲜重,最高杨桃的总酚含量为最低鳄梨的8.78倍;ORAC和FRAP抗氧化值分别为607.05～2631.17μmol TE/100 g鲜重和462.12～1067.92μmol TE/100 g鲜重,杨桃具有最高的ORAC和FRAP抗氧化值,分别是最低木瓜的4.33倍和鳄梨的2.31倍;11种水果提取物抑制肿瘤细胞HepG2增殖的IC50值为31.79～66.93 mg/mL,抑制活性最弱木瓜的IC50值为最强杨桃的2.11倍。水果提取物的总酚含量与其ORAC抗氧化值(R2=0.7839)、FRAP抗氧化值(R2=0.7636)和抑制HepG2细胞增殖的IC50值(R2=0.8847)之间具有显著的相关性。

引用

页码：2383 / 2387

页数：5

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