另一类型内皮祖细胞移植在损伤血管内膜修复中的作用

被引：3

作者：

蔡雪黎

肖方毅

张怀勤

黄伟剑

林以诺

周浩

林杰

杨德业

机构：

[1] 温州医学院附属第一医院心血管内科

来源：

吉林大学学报(医学版) | 2007年 / 04期

关键词：

内皮祖细胞; 细胞移植; 再内皮化; 兔;

D O I：

10.13481/j.1671-587x.2007.04.009

中图分类号：

R543 [血管疾病];

学科分类号：

1002 ; 100201 ;

摘要：

目的:观察体外培养的兔外周血另一种内皮祖细胞(EOCs)自体移植修复内膜球囊损伤血管的效果。方法:实验动物随机分为EOCs移植组(n=8)和对照组(n=8)。密度梯度离心法分离兔外周血单核细胞(MNCs),在EGM-2培养基的诱导分化下获得EOCs。将第二次传代的EOCs重悬于100μL生理盐水中移植至兔颈总动脉内膜球囊损伤血管局部(移植组),仅注入100μL生理盐水者作为对照组。同时用CM-DiI标记EOCs移植,进行细胞示踪。术后4周,荧光显微镜下观察标记细胞的分布,并测量内膜损伤血管段再生内皮覆盖率(RA/TA)和新生内膜增生程度(IA/MA)。结果:术后4周,荧光显微镜下,在血管中膜、新生内膜层和内膜表面均可见荧光标记的细胞。伊文思蓝染色显示,EOCs移植组的再生内皮覆盖率(89.1%±6.3%)显著高于对照组(62.1%±7.5%,P<0.01);与对照组比较,EOCs移植组的新生内膜形成明显减少,两者的IA/MA比值分别为0.88±0.14和0.44±0.06(P<0.01)。结论:EOCs移植可显著加速内膜损伤血管的再内皮化,并减少新生内膜的形成。

引用

页码：662 / 666+777 +777

页数：6

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