EST分子标记开发研究进展

被引：23

作者：

陈全求

詹先进

蓝家样

黄云

机构：

[1] 湖北省农业科学院经济作物研究所

来源：

中国农学通报 | 2008年 / 09期

关键词：

EST; 分子标记; 开发;

D O I：

暂无

中图分类号：

Q943.2 [植物基因工程];

学科分类号：

071007 ; 090102 ;

摘要：

EST(expressed sequence tags)计划作为植物基因组计划的一个重要组成部分,已经在多种植物物种中开展起来,EST资源库的不断扩充为利用这些数据来开发EST分子标记奠定了基础。概括介绍了几种主要EST标记(EST-SSR,EST-PCR,EST-SNP,EST-AFLP,EST-RFLP)的开发策略,同时也提出了EST标记开发存在的一些问题及解决方法。

引用

页码：72 / 77

页数：6

共 23 条

[1] 白菜EST-SSR标记的通用性 [J].

忻雅 ;

崔海瑞 ;

张明龙 ;

姚艳玲 ;

卢美贞 ;

金基强 ;

林容杓 ;

崔水莲 .

细胞生物学杂志, 2006, (02) :248-252

[2] EST规模测序在家蚕功能基因组中的应用 [J].

邱咏梅 ;

夏庆友 .

蚕学通讯, 2002, (04) :16-19

[3] Characterisation of single nucleotide polymorphisms in sugarcane ESTs [J].

Cordeiro, GM ;

Eliott, F ;

McIntyre, CL ;

Casu, RE ;

Henry, RJ .

THEORETICAL AND APPLIED GENETICS, 2006, 113 (02) :331-343

[4] Quantifying novel sequence variation and selective advantage in synthetic hexaploid wheats and their backcross-derived lines using SSR markers [J].

Zhang, PZ ;

Dreisigacker, S ;

Melchinger, AE ;

Reif, JC ;

Kazi, AM ;

Van Ginkel, M ;

Hoisington, D ;

Warburton, ML .

MOLECULAR BREEDING, 2005, 15 (01) :1-10

[5]

Single nucleotide polymorphism, haplotype diversity and recombination in the Isa gene of barley[J] . P. C. Bundock,R. J. Henry.Theoretical and Applied Genetics . 2004 (3)

[6]

Snipping polymorphisms from large EST collections in barley （ Hordeum vulgare L.）[J] . R. Kota,S. Rudd,A. Facius,G. Kolesov,T. Thiel,H. Zhang,N. Stein,K. Mayer,A. Graner.Molecular Genetics and Genomics . 2003 (1)

[7] Development and transferability of apricot and grape EST microsatellite markers across taxa [J].

Decroocq, V ;

Favé, MG ;

Hagen, L ;

Bordenave, L ;

Decroocq, S .

THEORETICAL AND APPLIED GENETICS, 2003, 106 (05) :912-922

[8] Single nucleotide polymorphisms in cytochrome P450 genes from barley [J].

Bundock, PC ;

Christopher, JT ;

Eggler, P ;

Ablett, G ;

Henry, RJ ;

Holton, TA .

THEORETICAL AND APPLIED GENETICS, 2003, 106 (04) :676-682

[9]

Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley （Hordeum vulgare L.）[J] . T. Thiel,W. Michalek,R. Varshney,A. Graner.Theoretical and Applied Genetics . 2003 (3)

[10]

Direct selection of expressed sequences on a YAC clone revealed proline-rich-like genes and BARE-1 sequences physically linked to the complex Mla powdery mildew resistance locus of barley （ Hordeum vulgare L.）[J] . Gerhard Schwarz,Wolfgang Michalek,Ahmed Jahoor,Volker Mohler.Plant Science . 2002 (2)

← 1 2 3 →