Community Diversity of Associated Bacteria of Radopholus similis

被引:2
作者
Fen ZHENG [1 ,2 ]
Meiying FU [2 ]
Zhixiang ZHAO [2 ]
Miancai CHEN [2 ]
机构
[1] College of Environment and Plant Protection, Hainan University
[2] Key Laboratory of Plant Diseases and Insect Pests Integrated Control of Hainan Province, Agricultural Environment and Plant Protection Research Institute of Hainan Academy of Agricultural Sciences
关键词
Radopholus similis; Associated bacteria; ARDRA; Phylogenetic analysis; China;
D O I
10.19579/j.cnki.plant-d.p.2012.02.009
中图分类号
S436.68 [多年生草本果类病虫害];
学科分类号
090401 ; 090402 ;
摘要
[Objective] The paper was to reveal the species abundance of associated bacterial community of Radopholus similis, and the diversity of its bacteria. [Methods] Through the construction of 16S rDNA cloning library of bacteria, the positive clone was carried out ARDRA analysis, and the phylogenetic tree was constructed. [Results] OUT (Operational Taxonomic Units) was classified according to sequences similarity of 97%, and the constructed 16S rDNA library of bacteria contained 13 OTUs, belonging to Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria, respectively. The dominant species was Betaproteobacteria, especially Burkholderia was the dominant bacterium. [Conclusions] The diversity of associated bacteria of R. similis was high, and these bacteria may have certain ecological significance to R. similis.
引用
收藏
页码:33 / 37
页数:5
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