对虾白斑综合症病毒囊膜蛋白VP19的融合表达及其抗病毒感染作用

被引：13

作者：

贾启军

孟小林

徐进平

王健

鲁伟

机构：

[1] 武汉大学生命科学学院病毒学国家重点实验室

来源：

Virologica Sinica | 2006年 / 06期

关键词：

对虾白斑综合症病毒(WSSV); VP19; 抗WSSV感染;

D O I：

暂无

中图分类号：

S945.4 [各种虾的病虫害及其防治];

学科分类号：

0906 ;

摘要：

根据GenBank上WSSV囊膜蛋白基因vp19的序列,设计并合成引物,PCR扩增得到vp19基因并克隆到pGEM‐T载体中,经过BamHⅠ/HindⅢ酶切、连接并将vp19插入到pET32b表达载体中。用重组质粒pET32b-vp19转化大肠杆菌Origam(iDE3)pLysS,在IPTG诱导下,融合蛋白Trx-VP19以可溶性的形式得到表达,经SDS-PAGE和Western-blot检测显示其分子量与预期的大小相符合。目的蛋白经Ni2+柱纯化并定量后分别直接注射鳌虾和包被饲料投喂鳌虾。实验结果表明注射Trx-VP19可以提高鳌虾个体抗WSSV感染力的作用。

引用

页码：585 / 588

页数：4

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