利用SSR标记技术分析中国啤酒大麦品种的遗传多样性

被引：20

作者：

张大乐

高红云

李锁平

机构：

[1] 河南大学农业生物技术研究所

来源：

西北农业学报 | 2007年 / 03期

关键词：

啤酒大麦; SSR; 遗传多样性; 聚类分析;

D O I：

暂无

中图分类号：

S512.31 [];

学科分类号：

摘要：

利用SSR标记技术对中国38个啤酒大麦品种的遗传背景进行了遗传多样性分析。结果表明,所用28对SSR引物(在大麦7对染色体长、短臂位置各选用了2对)中,有26对SSR引物有多态性,共检测出187个位点,其中172个等位位点有多态性,占91.9%;每个引物可扩增出1～22个位点,平均每个引物产生6.6个等位位点。26对SSR引物的多态性信息含量PIC最高为0.995,最低为0.375,平均PIC为0.713。聚类结果表明,这些品种的遗传距离(GD)聚类范围较小,分布范围在0.01124～0.74510,在遗传距离GD值0.6水平上,这些品种聚成了2大类,下分4个亚类。SSR标记揭示出这38个啤酒大麦品种遗传变异较小,遗传基础比较狭窄。

引用

页码：72 / 76

页数：5

共 8 条

[1] 我国大麦生产、需求与育种对策 [J].

杨建明 ;

沈秋泉 ;

汪军妹 ;

朱靖环 .

大麦科学, 2003, (01) :1-6

[2]

Assessment of genetic diversity within and among Basmati and non-Basmati rice varieties using AFLP, ISSR and SSR markers[J] . Navinder Saini,Neelu Jain,Sunita Jain,Rajinder K. Jain.Euphytica . 2004 (3)

[3]

Characterization of a flint maize （ Zea mays var. indurata ） Italian landrace, II. Genetic diversity and relatedness assessed by SSR and Inter-SSR molecular markers[J] . G. Barcaccia,M. Lucchin,P. Parrini.Genetic Resources and Crop Evolution . 2003 (3)

[4] Temporal flux in the morphological and molecular diversity of UK barley [J].

Koebner, RMD ;

Donini, P ;

Reeves, JC ;

Cooke, RJ ;

Law, JR .

THEORETICAL AND APPLIED GENETICS, 2003, 106 (03) :550-558

[5]

Genomic analysis of cultivated barley （Hordeum vulgare） using sequence-tagged molecular markers. Estimates of divergence based on RFLP and PCR markers derived from stress-responsive genes, and simple-sequence repeats （SSRs）[J] . E. Maestri,A. Malcevschi,A. Massari,N. Marmiroli.Molecular Genetics and Genomics . 2002 (2)

[6]

The use of microsatellite markers for detection of genetic diversity in barley populations[J] . D. Struss,J. Plieske.TAG Theoretical and Applied Genetics . 1998 (1-2)

[7] RAPD variation in wild populations of four species of the genus Hordeum (Poaceae) [J].

De Bustos, A ;

Casanova, C ;

Soler, C ;

Jouve, N .

THEORETICAL AND APPLIED GENETICS, 1998, 96 (01) :101-111

[8]

An evaluation of the utility of SSR loci as molecular markers in maize （Zea mays L.）: comparisons with data from RFLPS and pedigree[J] . J. S. C. Smith,E. C. L. Chin,H. Shu,O. S. Smith,S. J. Wall,M. L. Senior,S. E. Mitchell,S. Kresovich,J. Ziegle.TAG Theoretical and Applied Genetics . 1997 (1-2)

← 1 →