工程菌产黑色素的抗氧化作用研究

被引:19
作者
李晓燕
刘志洪
王鹏
沈萍
蔡汝秀
机构
[1] 武汉大学化学与分子科学学院
[2] 武汉大学化学与分子科学学院 湖北武汉
[3] 湖北武汉
关键词
苏云金芽孢杆菌(Bacillusthuringiensis); 黑色素; 羟自由基;
D O I
10.14188/j.1671-8836.2003.06.005
中图分类号
Q936 [微生物生物化学];
学科分类号
071010 ;
摘要
利用黑色素对苯甲酸羟基化产物荧光强度的猝灭作用,研究了重组的苏云金芽孢杆菌(Bacillusthuringiensis)pHTAM菌株产生的黑色素对羟自由基的清除作用,发现其具有与Sigma标准黑色素相当的清除羟基自由基的能力,且优于生物提取的国产商品黑色素;与硫脲等其它常见的抗氧化剂比较,该工程菌产黑色素清除羟自由基的效果明显,尤其是在低剂量时这种优势更为突出.通过动力学研究证实其对羟自由基的清除作用是基于氧化还原反应而非吸附作用.
引用
收藏
页码:693 / 696
页数:4
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