The simultaneous determination of total nitrogen (TN) and total phosphorus (TP) in seawater by micro-autoana- lyzer after persulphate oxidation was made. The relative error is less than 3. 8 % when TN is higher than 17. 7μmol/L and less than 5. 8 % when TP is higher than 0. 38 μmol/L. The recovery percentage of TN and TP are 96 %-123 % and 94 %-113 %. During iced conservation after persulphate oxidation, the concentrations of TN and TP did not change with time.