单细胞凝胶电泳技术的研究进展及其应用

被引:15
作者
王小红
江洪
夏明珠
杨益寿
机构
[1] 湖北医科大学一附院生育门诊!武汉
关键词
单细胞凝胶电泳; DNA损伤;
D O I
暂无
中图分类号
R446 [实验室诊断];
学科分类号
100208 ;
摘要
单细胞凝胶电泳技术是一种快速、敏感、简便、廉价的在单细胞水平上检测真核细胞DNA损伤与修复的方法。本文总结了该实验技术的发展、基本原理、检测分析方法、优点及其应用前景。
引用
收藏
页码:5 / 7
页数:2
相关论文
共 14 条
[1]  
Comparing the genotoxic sensitivities of human peripheral blood lymphocytes and mucosa cells of the upper aerodigestive tract using the Comet assay. Kleinsasser NH,Wallner BC,Kastenbauer ER,et al. Mutation Research . 2000
[2]  
Psoriasis patients with basal cell carcinoma have more repair-mediated DNA strand-breaks after UVC damage in lymphocytes than psoriasis patients without basal cell carcinoma. Moller P,Wallin H,Dybdahl M,et al. Cancer Letters . 2000
[3]  
Evaluation of DNA damage by the comet assay in shoe workers exposed to toluene and other organic solvents. Pitargue M,Vaglenov A,Nosko M,et al. Mutation Research . 1999
[4]  
Comparison of chemically induced DNA breakage in cellular and subcellular systems using the comet assay. Kasamatsu T,Kohda K,Kawazoe Y. Mutation Research . 1996
[5]  
Testicular and sperm DNA damage after treatment with fludarabine for chronic lymphocytic leukaemia. Chatterjee R,Haines GA,Perera DM,et al. Human Reproduction . 2000
[6]  
In vivo DNA damage in gastric epithelial cells. Everett SM,White KL,Schorah CJ,et al. Mutation Research . 2000
[7]  
A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides. Singh NP,Tice RR,Stephens RE,et al. Mutation Research . 1991
[8]  
Fibroblast radio sensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters. Eastham AM,Marples B,Kiltie AE,et al. British Journal of Cancer . 1999
[9]  
Enhanced benzene-induced DNA damage in PMA-stimulated in vitro and in LPS-treated animals. Tuo J,Loft S and Poulsen HE. Free Radical Biology and Medicine . 1999
[10]  
Levels of peripheral blood cell DNA damage in insulin dependent diabetes mellitus human subject. Hannon-Fletcher Mp,O Kane MJ,Moles KW,et al. Mutation Research . 2000