bFGF和EGF对体外培养新生大鼠海马神经元促生长作用的研究

被引:6
作者
苏万东
吴承远
辛华
仇红
王建刚
机构
[1] 山东医科大学附属医院神经外科
[2] 山东医科大学细胞生物学教研室
关键词
碱性成纤维细胞生长因子; 表皮生长因子; 海马; 神经元; 体外培养;
D O I
暂无
中图分类号
R321.5 [人体部位胚胎学];
学科分类号
摘要
目的 研究不同浓度碱性成纤维细胞生长因子 (bFGF)和表皮生长因子 (EGF)对新生大鼠海马神经元生长活性的影响。方法 取新生大鼠海马组织进行原代体外分离培养 ,从细胞形态学方面及应用MTT法观察bFGF和EGF对大鼠海马神经元的影响。结果 bFGF能明显促进体外培养大鼠海马神经元存活及突起生长 ,EGF也能促进其存活 ,尤以促进突起生长更为明显 ;bFGF、EGF均能延缓其衰老 ,以bFGF最为显著 ;二者均能增强其细胞内琥珀酸脱氢酶的活性。结论 bFGF和EGF对体外培养新生大鼠海马神经元均有不同程度的促生长作用。
引用
收藏
页码:36 / 38
页数:3
相关论文
共 7 条
[1]  
Effects of basic fibroblast growth factor on neurons cultured from various regions of postnatal rat brain. Matsuda S,Saito H,Nishiyama N. Brain Research . 1990
[2]  
Effects of basic fibroblast growth factor on neurons cultured from various regions of postnatal rat brain. Matsuda S,Saito H,Nishiyama N. Brain Research . 1990
[3]  
Nuclear and cytoplasmic localization of basic fibroblast growth factor in astrocytes and CA2 hippocampal neurons. Woodward WR,Nishi R,Meshul CK,et al. The Journal of Nutrition . 1992
[4]  
Epidermal growth factor and basic fibroblast growth factor: effects on an overlapping poplation of neocortical neurons in vitro. Korublum HI,Raymon Hk,Morrison RS,et al. Brain Research . 1990
[5]  
Selective and nonseletive stimulation of central cholinergic and dopaminergic development in vitro by nerve growth factor, basic fibroblast growth factor, epidermal growth factor, insulin and the insulin-like growth factor I and Ⅱ. Knusel B,Michel PP,Schwaber JS,et al. The Journal of Neuroscience . 1990
[6]  
Neurotrophic effects of basic and acidic fibroblast growth factors are not mediated through glial cells. Walicke PA,Baird A. Brain Research . 1998
[7]  
Regional distribution and developmental expression of epidermal growth factor and transforming growth factor-alpha mRNA in mouse brain by a quantitative nuclease protection assay. Lazar LM,Blum M. The Journal of Neuroscience . 1992