bFGF和EGF对体外培养新生大鼠海马神经元促生长作用的研究

被引：6

作者：

苏万东

吴承远

辛华

仇红

王建刚

机构：

[1] 山东医科大学附属医院神经外科

[2] 山东医科大学细胞生物学教研室

来源：

中华神经外科杂志 | 2000年 / 02期

关键词：

碱性成纤维细胞生长因子; 表皮生长因子; 海马; 神经元; 体外培养;

D O I：

暂无

中图分类号：

R321.5 [人体部位胚胎学];

学科分类号：

摘要：

目的　研究不同浓度碱性成纤维细胞生长因子 (bFGF)和表皮生长因子 (EGF)对新生大鼠海马神经元生长活性的影响。方法　取新生大鼠海马组织进行原代体外分离培养 ,从细胞形态学方面及应用MTT法观察bFGF和EGF对大鼠海马神经元的影响。结果　bFGF能明显促进体外培养大鼠海马神经元存活及突起生长 ,EGF也能促进其存活 ,尤以促进突起生长更为明显 ;bFGF、EGF均能延缓其衰老 ,以bFGF最为显著 ;二者均能增强其细胞内琥珀酸脱氢酶的活性。结论　bFGF和EGF对体外培养新生大鼠海马神经元均有不同程度的促生长作用。

引用

页码：36 / 38

页数：3

共 7 条

[1]

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[2]

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[3]

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[6]

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