茶园土壤微生物群落基因多样性

被引:34
作者
薛冬
姚槐应
黄昌勇
机构
[1] 浙江大学环境与资源学院
关键词
茶园土壤; 微生物群落; 16S rDNA; DGGE;
D O I
10.13287/j.1001-9332.2007.0142
中图分类号
S571.1 [茶];
学科分类号
0902 ; 090203 ;
摘要
应用PCR技术,直接从土壤中抽提总DNA,扩增16S rDNAV3片段,采用变性梯度凝胶电泳(DGGE)分析16S rDNAV3片段的多态性,研究了杭州西湖梅家坞不同植茶年龄(8、50和90年)、不同利用方式(茶园、荒地和林地)的土壤微生物群落基因多样性.结果表明:不同植茶年龄和不同土地利用方式影响土壤微生物群落的基因多样性.荒地、茶园和林地土壤微生物群落基因多样性指数明显不同(P<0.05),其排列顺序为荒地>茶园>林地.不同植茶年龄的土壤中,50年茶园土壤的微生物群落基因多样性指数、微生物量碳和基础呼吸明显高于8年和90年茶园土壤(P<0.05).
引用
收藏
页码:843 / 847
页数:5
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