拟南芥雄性不育突变体ms1142的遗传定位与功能分析

被引:6
作者
常玉花
周鹊
杨仲南
张森
机构
[1] 上海师范大学生命与环境科学学院
关键词
花药发育; 拟南芥; 雄性不育突变体; 图位克隆;
D O I
暂无
中图分类号
Q943 [植物细胞遗传学];
学科分类号
071007 ; 090102 ;
摘要
经EMS诱变野生型拟南芥(Arabidopsis thaliana)群体筛选得到一株雄性不育突变体ms1142,突变体的果荚短小,不含种子。细胞学观察和扫描电镜结果表明,突变体花药发育过程中,花药中小孢子外壁异常、破裂,最后没有花粉形成。遗传分析表明,该突变体为隐性单核基因突变所致;利用图位克隆的方法将MS1142基因定位于第1条染色体的BAC克隆F16P17上44kb区间内,目前尚未见该区间内有雄性不育基因的报道。以上结果结合生物信息学分析表明,MS1142是一个新的调控花药发育的关键基因。该工作为花药发育关键基因MS1142的克隆及功能分析奠定了基础。
引用
收藏
页码:404 / 410
页数:7
相关论文
共 11 条
[1]   水稻OsMS2基因在花药发育中的功能分析 [J].
方子君 ;
石其龙 ;
杨仲南 ;
张森 .
植物学通报, 2008, 25 (06) :665-672
[2]   拟南芥雄性不育突变体ms1502的遗传及定位分析 [J].
易君 ;
高菊芳 ;
张在宝 ;
江华 ;
周根余 ;
杨仲南 ;
张森 .
云南植物研究, 2006, (03) :283-288
[3]  
RUPTURED POLLEN GRAIN1, a Member of the MtN3/saliva Gene Family, Is Crucial for Exine Pattern Formation and Cell Integrity of Microspores in Arabidopsis1[C][W][OA][J] . Guan, Yue-Feng,Huang, Xue-Yong,Zhu, Jun,Gao, Ju-Fang,Zhang, Hong-Xia,Yang, Zhong-Nan.Plant Physiology . 2008 (2)
[4]   Callose synthase (CalS5) is required for exine formation during microgametogenesis and for pollen viability in Arabidopsis [J].
Dong, XY ;
Hong, ZL ;
Sivaramakrishnan, M ;
Mahfouz, M ;
Verma, DPS .
PLANT JOURNAL, 2005, 42 (03) :315-328
[5]  
Disruption of the novel plant protein NEF1 affects lipid accumulation in the plastids of the tapetum and exine formation of pollen, resulting in male sterility in Arabidopsis thaliana[J] . TohruAriizumi,KatsunoriHatakeyama,KokichiHinata,RieInatsugi,IkuoNishida,ShuseiSato,TomohikoKato,SatoshiTabata,KinyaToriyama.The Plant Journal . 2004 (2)
[6]   Resources for targeted insertional and deletional mutagenesis in Arabidopsis [J].
Zhang, S ;
Raina, S ;
Li, H ;
Li, J ;
Dec, E ;
Ma, H ;
Huang, H ;
Fedoroff, NV .
PLANT MOLECULAR BIOLOGY, 2003, 53 (01) :133-150
[7]  
A novel male-sterile mutant of Arabidopsis thaliana , faceless pollen-1 , produces pollen with a smooth surface and an acetolysis-sensitive exine[J] . Tohru Ariizumi,Katsunori Hatakeyama,Kokichi Hinata,Shusei Sato,Tomohiko Kato,Satoshi Tabata,Kinya Toriyama.Plant Molecular Biology . 2003 (1)
[8]   High-throughput DNA extraction method suitable for PCR [J].
Xin, ZG ;
Velten, JP ;
Oliver, MJ ;
Burke, JJ .
BIOTECHNIQUES, 2003, 34 (04) :820-+
[9]   Anther developmental defects in Arabidopsis thaliana male-sterile mutants [J].
Sanders, PM ;
Bui, AQ ;
Weterings, K ;
McIntire, KN ;
Hsu, YC ;
Lee, PY ;
Truong, MT ;
Beals, TP ;
Goldberg, RB .
SEXUAL PLANT REPRODUCTION, 1999, 11 (06) :297-322
[10]   Biogenesis and function of the lipidic structures of pollen grains [J].
Piffanelli, P ;
Ross, JHE ;
Murphy, DJ .
SEXUAL PLANT REPRODUCTION, 1998, 11 (02) :65-80