无色孔雀石绿免疫原的合成与鉴定

被引：3

作者：

王权 ^{[1
]}

李健 ^{[2
]}

郭德华 ^{[2
]}

龚鹏飞 ^{[1
]}

机构：

[1] 中国农业科学院上海兽医研究所兽医公共卫生研究室农业部动物寄生虫学重点开放实验室

[2] 上海出入境检验检疫局

来源：

中国兽医科学 | 2007年 / 06期

关键词：

无色孔雀石绿; 免疫原; 合成;

D O I：

10.16656/j.issn.1673-4696.2007.06.017

中图分类号：

S948 [水产药物学、药理学];

学科分类号：

0906 ;

摘要：

用化学方法合成了硝基无色孔雀石绿(NO2-LMG),还原为氨基无色孔雀石绿(NH2-LMG),经红外光谱扫描鉴定NH2-LMG为所需化合物。氨基无色孔雀石绿经重氮法处理后与兔血清白蛋白(RSA)载体蛋白相连,通过紫外分光光谱鉴定,偶联产物与其前体物相比吸收峰发生了飘移,说明偶联成功。通过动物免疫试验和ELISA抗体特异性试验对该人工合成抗原免疫原性和与无色孔雀石绿反应原性的鉴定,表明成功合成了无色孔雀石绿免疫原。

引用

页码：534 / 538

页数：5

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