甾醇TMS衍生物EI源质谱规律研究

被引：6

作者：

缪妙

徐继林

严小军

侯云丹

机构：

[1] 宁波大学应用海洋生物技术教育部重点实验室

来源：

分析测试学报 | 2008年 / S1期

基金：

浙江省自然科学基金;

关键词：

TMS derivatives of sterols; mass spectrometry; Symbodinium sp;

D O I：

暂无

中图分类号：

R284 [中药化学];

学科分类号：

1008 ;

摘要：

The mass spectrometric fragmentation of TMS derivatives of sterols was studied and analyzed based on the mass spectrometry of the TMS derivatives of standards.The double bond numbers and the degree of the methylize,alkylize and the related position in the sterol nucleus and the side chains were confirmed by the characteristic ions.Total lipids were extracted using Bligh-Dyer method.Sterols were isolated by solvent partition with chloroform-hexane(1 ∶4,by volume),derivated by BSTFA,and analyzed by GC-MS.The regular pattern was used for identifying the sterols in a dinoflagellate from the Symbodinium sp.

引用

页码：59 / 62

页数：4