荧光定量PCR技术及其在临床上的应用

被引:21
作者
邓少丽
罗阳
机构
[1] 第三军医大学大坪医院检验科
[2] 第三军医大学大坪医院检验科 重庆
[3] 重庆
关键词
荧光定量; PCR; 病原体;
D O I
暂无
中图分类号
R446.9 [其他];
学科分类号
100208 ;
摘要
荧光定量PCR(FluorescenceQuantitivePolymeraseChainReactionFQ PCR)技术是在 90年代末期发展起来的一项全新技术。经过几年的实践及临床应用 ,已获得了较为稳定的检测效果。作为一种体外基因扩增技术 ,它能敏感地检测初始模板浓度及基因变异等情况。它的出现 ,克服了传统PCR技术普遍存在的假阳性及不能定量等问题 ,使得PCR技术更广泛地应用于临床 ,在监测患者病情、预后、指导用药等方面有着广阔的应用前景。本文概述了FQ PCR技术及其在临床上的应用 ,即常见病原体的检测[1,3 ] 及其在分子遗传学和法医学中的应用
引用
收藏
页码:301 / 303
页数:3
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