汉黄芩素增敏TNF促肿瘤细胞凋亡的实验研究

被引：5

作者：

杨岚 ^{[1
]}

王霞 ^{[2
]}

王琼 ^{[2
]}

孙虹 ^{[2
]}

机构：

[1] 成都中医药大学组织学与胚胎学教研室

[2] 四川大学华西第二附属医院分子与转化医学实验室

来源：

现代预防医学 | 2015年 / 42卷 / 01期

关键词：

TNF; 汉黄芩素; JNK; 抗肿瘤; 凋亡;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

100806 [中药药理学];

摘要：

目的研究JNK通路在汉黄芩素增敏TNF促进肺癌干细胞凋亡的作用机制。方法采用汉黄芩素和TNF单独或联合用药,LDH检测细胞凋亡,免疫蛋白印记(Western Bloting,WB)检测JNK磷酸化水平和凋亡蛋白的表达。结果汉黄芩素显著增强TNF诱导的抗肿瘤作用,并且这个作用通过促进TNF诱导的JNK通路的磷酸化来实现。同时伴随潜在的TNF诱导的caspase 8活化,引起凋亡的启动。凋亡抑制因子z VAD-fmk和JNK通路抑制因子SP600125均可以扭转这种凋亡。更为重要的是,汉黄芩素对于正常的支气管上皮细胞则不会出现增敏TNF诱导细胞凋亡的作用。结论汉黄芩素可能通过促进JNK通路磷酸化增敏TNF抗肺癌作用。

引用

页码：117 / 120

页数：4

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