用RAPD、ISSR和AFLP标记分析系谱关系明确的甘薯品种的亲缘关系

被引：47

作者：

贺学勤

刘庆昌

翟红

王玉萍

机构：

[1] 中国农业大学农业部作物基因组学与遗传改良重点开放实验室

来源：

作物学报 | 2005年 / 10期

基金：

国家杰出青年科学基金;

关键词：

甘薯; RAPD; ISSR; AFLP; 亲缘关系;

D O I：

暂无

中图分类号：

S531 [甘薯（红薯）];

学科分类号：

摘要：

用RAPDI、SSR和AFLP标记对系谱关系明确的7个甘薯品种进行了亲缘关系分析。24个RAPD引物、14个ISSR引物和9对AFLP引物分别扩增出1731、74和168条多态性带。3种分子标记在检测甘薯品种间遗传差异上相关程度高,其中RAPD与ISSR之间的相关系数最大为0.9328。用ISSR标记估计的品种间遗传距离为0.1286～1.0932,平均0.4883,大于其余2个标记的估计值。3种分子标记皆可揭示甘薯品种的亲缘关系,其中ISSR标记产生的聚类图与系谱图最吻合,认为ISSR标记更适于分析甘薯品种的亲缘关系。

引用

页码：1300 / 1304

页数：5

共 5 条

[1]

AFLP assessment of diversity in sweetpotato from Latin America and the Pacific region: Its implications on the dispersal of the crop[J] . Dapeng Zhang,Genoveva Rossel,Albert Kriegner,Robert Hijmans.Genetic Resources and Crop Evolution . 2004 (2)

[2]

The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin[J] . M. Fernández,A. Figueiras,C. Benito.Theoretical and Applied Genetics . 2002 (5)

[3]

Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon[J] . J. Garcia-Mas,M. Oliver,H. Gómez-Paniagua,M.C. de Vicente.TAG Theoretical and Applied Genetics . 2000 (5-6)

[4]

Assessing genetic diversity of sweet potato （Ipomoea batatas （L.） Lam.） cultivars from tropical America using AFLP[J] . Dapeng Zhang,Jim Cervantes,Zosimo Huamán,Edward Carey,Marc Ghislain.Genetic Resources and Crop Evolution . 2000 (6)

[5] Genetic diversity and relationships of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA [J].

Huang, JC ;

Sun, M .

THEORETICAL AND APPLIED GENETICS, 2000, 100 (07) :1050-1060

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