用RNAi沉默survivin基因表达对前列腺癌细胞系PC3的影响

被引：3

作者：

沈建军

刘家云

苏明权

缪应业

张青

郝晓柯

机构：

[1] 第四军医大学西京医院检验科

来源：

中国肿瘤生物治疗杂志 | 2006年 / 01期

关键词：

RNA干扰; Ssurvivin; 凋亡; 前列腺癌细胞;

D O I：

暂无

中图分类号：

R737.25 [前列腺肿瘤];

学科分类号：

100214 ;

摘要：

目的:运用RNA干扰(RNA interference,RNAi)技术阻断前列腺癌细胞系PC3中survivin基因的表达,并研究 survivin基因沉默后对PC3细胞产生的影响。方法:用真核转录载体pSilencerTM3．1-H1 neo构建针对survivin基因的重组真核转录载体pSilencer 3．1-SVV1、pSilencer 3．1-SVV2和pSilencer 3．1-SVV3,并用脂质体法转染前列腺癌细胞系PC3,通过RT- PCR、蛋白印迹实验检测sunrivin的表达变化,并用流式细胞术、MTT法检测转染后PC3细胞的凋亡、细胞周期、细胞生长速度、对顺铂的敏感性等芳面的变化。结果:重组载体pSilencer 3．1-SVV2和pSilencer 3．1-SVV3有效地阻断了PC3细胞中sur- vivin基因在mRNA和蛋白水平上的表达(P<0．01)。重组载体转染PC3细胞后,与对照组相比,细胞的凋亡增加了10％- 15％;细胞生长速度明显变慢,其细胞数在84 h时与对照组相比减少约30％;G1期细胞增加了10％,G2期和S期细胞减少了 5％以上。加入顺铂后,pSilencer 3．1-SVV2、pSilencer 3．1-SVV3重组质粒转染的PC3细胞的存活数下降了35％-45％,细胞凋亡则增加了10％-14％。结论:初步证明了survivin基因在前列腺癌细胞分化增殖、抗凋亡等方面所扮演的重要角色,为进一步阐明survivin基因与前列腺癌的关系以及以survivin基因为靶点的前列腺癌基因治疗研究奠定了基础。

引用

页码：37 / 41

页数：5

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