我国单纯疱疹病毒Ⅰ型168株糖蛋白D基因的克隆及序列分析

被引:11
作者
杨雄虎
侯云德
机构
[1] 病毒基因工程国家重点实验室,病毒基因工程国家重点实验室北京,北京
关键词
单纯疱疹病毒Ⅰ型; 糖蛋白D(gD); 多聚酶链反应(PCR);
D O I
10.13242/j.cnki.bingduxuebao.000068
中图分类号
学科分类号
摘要
依据HSV-1 Patton株糖蛋白D全基因序列,设计、合成一对引物,利用多聚酶链反应(PCR),从我国HSV-1 168株基因组DNA中扩增、克隆出糖蛋白D全基因序列,共1185bp,编码395个氨基酸,并将其推测的氨基酸序列同国外HSV-1 HFEM、SCl6、17和Patton株的糖蛋白D的氨基酸序列进行了详细比较,发现最保守的区域主要是除信号肽以外的胞外编码区。氨基酸的变异主要分布在信号肽区、跨膜区及胞浆内编码区。本工作对研究HSV-1糖蛋白D的结构和功能以及研制我国HSV-1基因工程疫苗都具重要意义。
引用
收藏
页码:308 / 315
页数:8
相关论文
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