生物发光共振能量转移技术及其应用

被引：9

作者：

李雅林 ^{[1
,2
]}

白波 ^{[3
]}

陈京 ^{[1
,3
]}

机构：

[1] 泰山医学院基础部神经生物学实验室

[2] 山东农业大学生命科学学院

[3] 济宁医学院

来源：

中国生物化学与分子生物学报 | 2009年 / 25卷 / 12期

关键词：

生物发光共振能量转移; 蛋白质-蛋白质相互作用; G蛋白偶联受体; 药物发现;

D O I：

10.13865/j.cnki.cjbmb.2009.12.002

中图分类号：

Q63 [生物光学];

学科分类号：

摘要：

生物发光共振能量转移(BRET)技术是近10年来出现的一种新的检测蛋白质-蛋白质相互作用的技术.它的最大优势是能在活细胞中实时进行检测,因此能够进行相互作用动力学的研究.本文系统阐述了BRET的原理和方法,综述了BRET技术的最新进展,以及该技术在G蛋白偶联受体(GPCRs)信号转导及药物发现中的应用.

引用

页码：1077 / 1082

页数：6

共 16 条

[1] 从近年Nobel奖项看生命科学进展 [J].

倪菊华 ;

周爱儒 .

中国生物化学与分子生物学报, 2009, 25 (05) :393-399

[2]

Physiological relevance of GPCR oligomerization and its impact on drug discovery[J] . Rosemarie Panetta,Michael T. Greenwood. &nbspDrug Discovery Today . 2008 (23)

[3]

Extended bioluminescence resonance energy transfer (eBRET) for monitoring prolonged protein–protein interactions in live cells[J] . Kevin D.G. Pfleger,Jasmin R. Dromey,Matthew B. Dalrymple,Esther M.L. Lim,Walter G. Thomas,Karin A. Eidne. &nbspCellular Signalling . 2006 (10)

[4]

Recruitment of β-arrestin2 to the dopamine D2 receptor: Insights into anti-psychotic and anti-parkinsonian drug receptor signaling[J] . &nbspNeuropharmacology . 2008 (8)

[5] Bioluminescence and chemiluminescence in drug screening [J].

Roda, A ;

Guardigli, M ;

Pasini, P ;

Mirasoli, M .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2003, 377 (05) :826-833

[6] Applications of bioluminescence- and fluorescence resonance energy transfer to drug discovery at G protein-coupled receptors [J].

Milligan, G .

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2004, 21 (04) :397-405

[7] The c-terminus of GRK3 indicates rapid dissociation of G protein heterotrimers [J].

Hollins, Bettye ;

Kuravi, Sudhakiranmayi ;

Digby, Gregory J. ;

Lambert, Nevin A. .

CELLULAR SIGNALLING, 2009, 21 (06) :1015-1021

[8]

Subramanian,C,Xu,Y,Johnson,CH,von,Arnim,AG.In vivo detection of protein–protein interaction in plant cells using BRET. Methods in Molecular Biology . 2004

[9]

Xu,Y,Piston,DW,Johnson,CH.A bioluminescence resonance energy transfer (BRET) system: application to interacting circadian clock proteins. Proceedings of the National Academy of Sciences of the United States of America . 1999

[10]

Angers,S,Salahpour,A,Joly,E,Hilairet,S,Chelsky,D,Dennis,M,Bouvier,M.Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET). Proceedings of the National Academy of Sciences of the United States of America . 2000

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