吴茱萸碱诱导人子宫颈癌HeLa细胞凋亡的机制研究

被引:12
作者
费晓方
王本祥
池岛乔
机构
[1] 吉林大学生命科学学院,吉林天然药物研究所,沈阳药科大学中日医药研究所吉林长春,吉林长春,辽宁沈阳
关键词
吴茱萸碱; HeLa细胞; 细胞凋亡;
D O I
10.16438/j.0513-4870.2002.09.001
中图分类号
R737.33 [子宫肿瘤];
学科分类号
100214 ;
摘要
目的 研究吴茱萸碱 (evodiamine)诱导人子宫颈癌HeLa细胞凋亡的分子生物学机制。方法 用结晶紫法和琼脂糖凝胶电泳法以及用两种caspase的蛋白酶抑制剂测定细胞凋亡过程中caspase的信号传导路径。结果 吴茱萸碱可诱导HeLa细胞的细胞膜皱缩、细胞质浓集及凋亡小体的形式 ,并清晰可见以 1 80bp倍数裂解的DNA梯形电泳带的出现。抑制剂VAD fmk(caspase家族总抑制剂 )和DEVD fmk(caspase 3抑制剂 )能部分抑制HeLa细胞的凋亡。结论 Evodiamine诱导人宫颈癌HeLa细胞凋亡 ;caspasecascade信号传导路径与凋亡密切相关
引用
收藏
页码:673 / 676
页数:4
相关论文
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