高效液相色谱-同位素稀释质谱法测定血清中的苯丙氨酸

被引：4

作者：

武利庆

王晶

机构：

[1] 中国计量科学研究院生物能源环境所

来源：

分析测试学报 | 2007年 / S1期

关键词：

Serum; Phenylalanine; Isotope dilution mass spectrometry; Uncertainty;

D O I：

暂无

中图分类号：

R446.1 [生物化学检验、临床检验];

学科分类号：

100208 ;

摘要：

Phenylalanine in serum was determined by HPLC isotope dilution mass spectrometry method with D8 labeled phenylalanine as internal standard.Phenylalanine and its isotope labeled analogue were monitored at the transitions m/z=166→120 and 172→126 in multiple reaction monitoring (MRM) mode,respectively,which effectively eliminated the matrix effect.The RSD for 5 repeats measurement of the same sample was 0.5%.Uncertainty was evaluated,which showed the main uncertainty was from the purity assessment of phenylalanine reference material and the variation of method.The presented method can be applied into value assessment of reference material of phenylalanine in serum,and it can be also used for traceability establishment of the measurement of phenylalanine in serum.

引用

页码：98 / 99+103 +103

页数：3