棉花赤霉素不敏感矮化GID1同源基因的克隆和表达分析

被引:37
作者
董静
尹梦回
杨帆
赵娟
覃珊
侯磊
罗明
裴炎
肖月华
机构
[1] 西南大学生物技术中心/农业部生物技术和作物品质改良重点实验室
基金
国家高技术研究发展计划(863计划);
关键词
GID1; 棉花; 纤维发育; 表达特异性;
D O I
暂无
中图分类号
S562 [棉];
学科分类号
090302 [植物营养学];
摘要
作为赤霉素(GA)受体,GID1是其重要的信号传导组分。为研究GA在棉花发育(特别是纤维发育)中的作用,本文在EST序列的基础上克隆了6个棉花GID1同源基因(GhGID1-1~6)。多重序列分析表明,棉花GID1同源蛋白GhGID1-1~6与拟南芥和水稻的GID1蛋白高度同源,具有多个与GA和DELLA蛋白的结合位点以及激素敏感酯酶家族保守域HGG和GXSXG。定量RT-PCR分析表明,GhGID1-1~6基因在棉花不同器官和组织中表达水平有差异,其中GhGID1-1和GhGID1-2在花器官中高表达,GhGID1-4基因在纤维和根中优势表达。胚珠体外培养基中外加GA使GID1同源基因表达水平发生变化,GhGID1-1和GhGID1-2基因的表达水平明显受GA抑制。比较发现,在胚珠和纤维中优势表达的GID1同源基因不同,GID1基因表达水平随发育时期变化的趋势也不相同,表明棉花胚珠和纤维具有相对独立的GA信号识别系统。
引用
收藏
页码:1822 / 1830
页数:9
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