神经生长因子对颅脑损伤后神经细胞的保护作用

被引：16

作者：

周政

陈惠孙

张可成

杨辉

机构：

[1] 第三军医大学附属新桥医院神经外科

[2] 第三军医大学附属大坪医院野战外科研究所

[3] 第三军医大学附属新桥医院神经外科重庆

[4] 重庆

来源：

中华实验外科杂志 | 2004年 / 05期

关键词：

脑; 神经生长因子; 神经细胞;

D O I：

暂无

中图分类号：

R651.15 [];

学科分类号：

1002 ; 100210 ;

摘要：

目的　探讨神经生长因子 (NGF)对颅脑损伤后神经细胞的保护作用。方法　建立大鼠液压脑损伤模型 ,向其脑室灌注 7SNGF 5 μg ,观测其对脑组织超氧岐化酶 (SOD)、谷胱苷肽过氧化物酶 (GSH Px)和过氧化物酶 (CAT)活性、[Ca2 + ]i及细胞凋亡的影响。结果　 (1)伤后 3d ,NGF处理组SOD、GSH Px和CAT含量分别为 (198.96± 7.76)UN/ml、(1.2 1± 0 .0 4)UN/mg蛋白、(3 2 .5 8± 2 .0 1)UN/mg蛋白 ,显著高于单纯致伤组 (P <0 .0 1)。 (2 )伤后 2 4h ,NGF处理组 [Ca2 + ]i为 (878.5 6± 13 7.3 0 )nmol/L ,明显低于单纯致伤组 (972 .48± 14 6.66)nmol/L(P <0 .0 1)。 (3 )NGF处理组凋亡细胞显著少于单纯致伤组 (P <0 .0 1)。结论　NGF能增加颅脑损伤后脑组织中主要抗氧化酶的活性 ,降低 [Ca2 + ]i超载 ,对神经细胞有明显保护作用。

引用

页码：77 / 78

页数：2

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