血管外膜损伤后血管组织氧化应激与内膜病变

被引：7

作者：

汤月霞 ^{[1
]}

梁春 ^{[2
]}

刘永 ^{[2
]}

吴宗贵 ^{[2
]}

机构：

[1] 济南军区青岛第一疗养院

[2] 第二军医大学长征医院心血管内科

来源：

第二军医大学学报 | 2008年 / 08期

关键词：

血管外膜损伤; 氧化应激; NADPH氧化酶; 活性氧; 动脉粥样硬化;

D O I：

暂无

中图分类号：

R543.5 [动脉疾病];

学科分类号：

1002 ; 100201 ;

摘要：

目的:探讨NADPH氧化酶相关的氧化应激在外膜损伤致内膜病变中的作用。方法:选用纯种新西兰大白兔,采用胶原酶消化+钝性机械分离的方法建立血管外膜损伤动物模型,采用H-E染色观察外膜损伤血管的形态变化,实时荧光定量PCR(real-time quantitative PCR,RQ-PCR)技术检测外膜损伤后血管组织NADPH氧化酶亚单位p22phox、抗氧化酶HO-1的mRNA表达,荧光探针检测外膜损伤后血管组织活性氧(reactive oxygen species,ROS)的生成。结果:外膜损伤可致内膜增生性病变;外膜损伤导致p22phox/HO-1 mRNA表达明显升高,血管组织ROS产量增加。结论:血管外膜参与了内膜病变形成的病理过程;NADPH氧化酶活性升高导致的氧化应激可能是外膜损伤致内膜病变的机制之一。

引用

页码：912 / 916

页数：5

共 7 条

[1] 兔血管外膜对血管重构及收缩功能影响的初步观察 [J].

牟华明 ;

祝之明 ;

王海燕 ;

王利娟 .

生理学报, 2003, (03) :290-295

[2] Heme oxygenase-1 attenuates the cisplatin-induced apoptosis of auditory cells via down-regulation of reactive oxygen species generation [J].

Kim, Hyung-Jin ;

So, Hong-Seob ;

Lee, Jeong-Han ;

Lee, Jae-Hyung ;

Park, Channy ;

Park, Sung-Yeol ;

Kim, Yun-Ha ;

Youn, Myung-Ja ;

Kim, Se-Jin ;

Chung, Sang-Young ;

Lee, Kang-Min ;

Park, Raekil .

FREE RADICAL BIOLOGY AND MEDICINE, 2006, 40 (10) :1810-1819

[3]

NAD(P)H Oxidase 4 Mediates Transforming Growth Factor-β1–Induced Differentiation of Cardiac Fibroblasts Into Myofibroblasts[J] . Ioan Cucoranu,Roza Clempus,Anna Dikalova,Patrick J. Phelan,Srividya Ariyan,Sergey Dikalov,Dan Sorescu.Circulation Research . 2005 (9)

[4] Tyrosine kinase-mediated activation of NAD(P)H oxidase enhances proliferative capacity of diabetic vascular smooth muscle cells [J].

Jeong, HY ;

Son, SM ;

Kim, YK ;

Yun, MR ;

Lee, SM ;

Kim, CD .

LIFE SCIENCES, 2005, 76 (15) :1747-1757

[5] Expression of NADH/NADPH oxidase p22phox in human coronary arteries [J].

Azumi, H ;

Inoue, N ;

Takeshita, S ;

Rikitake, Y ;

Kawashima, S ;

Hayashi, Y ;

Itoh, H ;

Yokoyama, M .

CIRCULATION, 1999, 100 (14) :1494-1498

[6]

Increased NADH-Oxidase-Mediated Superoxide Production in the Early Stages of Atherosclerosis: Evidence for Involvement of the Renin-Angiotensin System[J] . Ascan Warnholtz,Georg Nickenig,Eberhard Schulz,Roland Macharzina,Jan Hinrich Brasen,Mikhail Skatchkov,Thomas Heitzer,Johannes P. Stasch,Kathy K. Griendling,David G. Harrison,Michael Bohm,Thomas Meinertz,Thomas Munzel.Circulation . 1999 (15)

[7]

Assembly and activation of the phagocyte NADPH oxidase. Specific interaction of the N-terminal Src homology 3domain of p47phox with p22phox is required for activation of the NADPH oxidase .2 Sumimoto H,Hata K,Mizuki K,et al. JBiol Chem . 1996

← 1 →