Effect of Modified Wuzi Yanzong Granule(加味五子衍宗颗粒)on Patients with Mild Cognitive Impairment from Oxidative Damage Aspect

<正>Objective:To observe the effects of modified Wuzi Yanzong Granule（加味五子衍宗颗粒,WYG）on memory function and the activity of serum superoxide dismutase（SOD）, malondialdehyde（MDA）levels,leukocyte mitochondrial DNA（mtDNA）deletion rate andβ-amyloid protein1-28(Aβ1-28)in patients with mild cognitive impairment（MCI）.Methods:Thirty-six patients with MCI were selected based on the internationally recognized Petersen's criteria,and equally and randomly assigned to two groups.The treated group was treated with WYG and the control group was treated with placebo for 3 months.In addition,20 healthy subjects were included in the study as the normal control group.Changes of memory function,SOD activity,MDA content,leukocyte mtDNA deletion rate and Aβ1-28content were observed before and after treatment.Results: Compared with the normal control group,the memory quotient and SOD activity in patients with MCI decreased significantly（P<0.01）,while MDA,Aβ1-28levels and the leukocyte mtDNA deletion rate increased significantly（P<0.01）.After treatment,levels of memory quotient and serum SOD activity increased while the serum MDA level,leukocyte mtDNA deletion rate and Aβ1-28level decreased in the treated group compared with those before treatment（P<0.01,P<0.05）.Meanwhile,leukocyte mtDNA deletion rate and Aβ1-28content in the treated group were all lower than those in the control group（P<0.05）.Conclusion:WYG could improve memory function in patients with MCI and the therapeutic mechanism is possibly related to the increased activity of anti-oxidase,the improved free radical metabolism and the alleviation of leukocyte mtDNA oxidation damage.WYG shows clinical significance in delaying the progression of MCI.